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ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Cellular Biochemistry
Volume 13 - 2025 |
doi: 10.3389/fcell.2025.1546073
This article is part of the Research Topic Mechanism study of natural bioactive molecules using omics technology View all articles
Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats
Provisionally accepted- 1 Beijing University of Chinese Medicine, Beijing, China
- 2 China Academy of Chinese Medical Sciences, Beijing, Beijing Municipality, China
Blood proteomics offers a powerful approach for identifying disease-specific biomarkers. However, no reliable blood markers are currently available for the diagnosis stroke. Nervonic acid (NA), a vital long-chain monounsaturated fatty acid found in mammalian nervous tissue, shows promising therapeutic potential in neurological disorders. This study aimed to develop a reliable methodology for whole blood proteomics to identify early warning biomarkers and evaluate drug treatment efficacy. After modeling via the classic thread embolization method, whole blood samples were collected from the rats. Morphological assessments of brain tissue indicated that NA significantly mitigated brain and neuronal damage in rats. The differential protein expression profile was analyzed using liquid chromatography -tandem mass spectrometry (LC-MS/MS) whole blood proteomics. Gene Ontology (GO) analysis revealed that, compared to ginkgo biloba extract (EGb), the proteins differentially expressed under NA intervention were predominantly involved in oxidative stress response and calcium-dependent adhesion processes. Key targets of NA in the treatment of middle cerebral artery occlusion (MCAO) models included ENO1, STAT3, NME2, VCL, and CCT3. This whole blood proteomic approach provides a comprehensive understanding of protein profiles associated with disease states, offering valuable insights into potential therapeutic targets and enabling the evaluation of NA and EGb intervention efficacy. Our findings underscore the protective effects of NA against cerebral ischemia-reperfusion injury and highlight its potential as a treatment for stroke.
Keywords: Whole blood proteome, Cerebral ischemia-reperfusion injury, nervonic acid, Ginkgo biloba extract, Mechanism
Received: 16 Dec 2024; Accepted: 06 Jan 2025.
Copyright: © 2025 Li, Zhang, Li and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xianyu Li, China Academy of Chinese Medical Sciences, Beijing, 100700, Beijing Municipality, China
Qing Wang, Beijing University of Chinese Medicine, Beijing, China
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