94% of researchers rate our articles as excellent or good
Learn more about the work of our research integrity team to safeguard the quality of each article we publish.
Find out more
ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Stem Cell Research
Volume 13 - 2025 | doi: 10.3389/fcell.2025.1527557
This article is part of the Research Topic Neural Stem Cell and Its Niche View all articles
Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate
Provisionally accepted
Klaudia Radoszkiewicz 1
Aleksandra Bzinkowska 1
Monika Sypecka 1
Dorota Sulejczak 1
Daniela Ferrari 2 Maurizio Gelati 3,4 Angelo Luigi Vescovi 2,3
Anna Sarnowska 1*- 1 Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland
- 2 Department of Biotechnology and Biosciences, School of Sciences, University of Milano-Bicocca, Milano, Lombardy, Italy
- 3 IRCCS Casa Sollievo della Sofferenza Hospital, San Giovanni Rotondo, Apulia, Italy
- 4 L'Unita Produttiva per terapia Avanzata, Unit Fondazione IRCCS Casa Sollievo della Sofferenza, Foggia, Italy
Human neural stem/progenitor cells (hNSCs) can potentially treat neurological diseases, but their low survival and proliferation rates after transplantation remain challenging. In our study, we preincubated hNSCs with the human cerebrospinal fluid (CSF) to obtain closer to the physiological brain environment and to assess NSC fate and their therapeutic abilities in vitro, ex vivo, and in vivo. We observed significant changes in the differentiation, migratory, and secretory potential of CSF-treated hNSCs, as well as their elevated neuroprotective potential after co-culture with ischemically damaged by oxygen-glucose deprivation (OGD) organotypic rat hippocampal slices culture (OHC) in comparison to the cells cultured in the standard conditions. Next, we investigated their survival and anti-inflammatory abilities in an in vivo ouabain-induced stroke model. This study highlighted and confirmed the critical importance of nutritional supplementation in maintaining NSC culture and enhancing its therapeutic properties.
Keywords: Neural Stem Cells, ischemic stroke, Cerebrospinal Fluid, Neuroprotection, cell therapy
Received: 13 Nov 2024; Accepted: 24 Feb 2025.
Copyright: © 2025 Radoszkiewicz, Bzinkowska, Sypecka, Sulejczak, Ferrari, Gelati, Luigi Vescovi and Sarnowska. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Anna Sarnowska, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.