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ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Cell Adhesion and Migration
Volume 13 - 2025 |
doi: 10.3389/fcell.2025.1512672
Interaction of Integrin αvβ3 and Fibronectin under Fluid Shear Forces: Implications for Tumor Cell Adhesion and Migration
Provisionally accepted- 1 School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China
- 2 Institute for Stroke and Dementia Research, LMU Munich University Hospital, Munich, Bavaria, Germany
- 3 First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi Province, China
The interaction between integrin αvβ3 and fibronectin enables tumor cell adherence to endothelial layers under diverse hydrodynamic blood flow conditions, particularly in low shear stress regions. Understanding the mechanical binding characteristics between integrin αvβ3 and fibronectin under different hydrodynamic environments can provide insights into tumor cell invasion and proliferation. Here, the adhesive behavior of fibronectin-functionalized microspheres on integrin αvβ3-coated substrates under various wall fluid shear forces (0.1-0.7 dyn/cm 2 ) was assessed using a parallel plate flow chamber system. The bond lifetimes of integrin αvβ3-fibronectin initially increased and then decreased, indicating transition from a "catch bond" to "slip bond." Upon perfusion of fibronectincoated microspheres into flow chambers with high-density integrin αvβ3 coating, the rolling velocity of the microspheres increased with increasing shear force. Additionally, the mean stop time and stop frequency exhibited a force-dependent biphasic pattern, initially increasing and then decreasing with shear force, demonstrating a nuanced response to mechanical forces. Thus, the integrin αvβ3fibronectin interaction displays a "catch bond" property, influencing cell distribution in varying fluid shear forces by promoting optimal adhesion in specific shear sites. These insights enhance our understanding of tumor cell adhesion and migration in hydrodynamic environments and may aid the design of integrin αvβ3-targeted therapies.
Keywords: Integrin αvβ3, fibronectin, Tumor cell adhesion, force, Catch bond
Received: 17 Oct 2024; Accepted: 24 Jan 2025.
Copyright: © 2025 Zhuo, Li, Yang, Li, Luo and Fengxia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Quhuan Li, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China
Zhang Fengxia, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi Province, China
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