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ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Membrane Traffic and Organelle Dynamics
Volume 13 - 2025 | doi: 10.3389/fcell.2025.1512238
This article is part of the Research Topic Plant and Probiotic Nanovesicles: Innovations and Applications View all articles
Plant and animal-derived fusion nanovesicles rescue inflammationcompromised osteogenic potential of periodontal ligament stem cells
Provisionally accepted- 1 Sun Yat-sen University, Shenzhen Campus, Shenzhen, Guangdong, China
- 2 Northwest A&F University, Xianyang, Shaanxi Province, China
- 3 Shenzhen Children's Hospital, Shenzhen, Guangdong Province, China
Periodontitis is a chronic inflammatory disease affecting the supporting tissues of the teeth and has emerged as a global public health issue. Current therapies primarily address pathogenic factors and alleviate symptoms, with limited options available for complete restoration and reconstruction of already absorbed periodontal bone tissue. In this study, we developed a nanotherapeutic strategy utilizing fusion nanovesicles (FVs) to modulate the inflammatory microenvironment and create a regenerative niche for periodontal ligament stem cells (PDLSCs), which play a crucial role in periodontal tissue repair. The FVs are composed of Scutellaria baicalensis nanovesicles (SBNVs) with anti-Porphyromonas gingivalis (P. gingivalis) and antiinflammatory properties, combined with PDLSC membrane-derived nanovesicles genetically engineered to express TNFR1. These FVs preserved the biological activity of SBNVs and the immunomodulatory function of PDLSCs. Additionally, FVs effectively captured and cleared TNF-α from the microenvironment through TNFR1. Moreover, FVs alleviated the inflammatory response of PDLSCs induced by P. gingivalis-LPS (Pg-LPS) and TNF-α, restoring their proliferation, migration, and osteogenic differentiation capabilities. Hence, this nanotherapeutic strategy holds great potential for treating periodontitis.
Keywords: Plant-derived nanovesicles, TNFR1, fusion nanovesicles, Inflammatory microenvironment, Periodontal ligament stem cells, osteogenic differentiation Abstract：
Received: 16 Oct 2024; Accepted: 05 Feb 2025.
Copyright: © 2025 Lin, Li, Wang, Lu, Xu, Chen and Dai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Quanle Xu, Northwest A&F University, Xianyang, 712100, Shaanxi Province, China
Hongbo Chen, Sun Yat-sen University, Shenzhen Campus, Shenzhen, Guangdong, China
Dongling Dai, Shenzhen Children's Hospital, Shenzhen, Guangdong Province, China
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