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ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Epigenomics and Epigenetics
Volume 13 - 2025 |
doi: 10.3389/fcell.2025.1443888
Overview of distinct 8-Oxoguanine profiles of messenger RNA in normal and senescent cancer cells
Provisionally accepted- 1 Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao, China
- 2 Nanfang Hospital, Southern Medical University, Guangzhou, China
Background: Cellular senescence plays a key role in the development of cancer, but the underlying mechanisms are unknown. Recently, several recent studies have shown that RNA methylation is closely related to cancer cell aging. 8-Oxoguanine (o 8 G) is an important and widely distributed methylation modification whose role in cancer cell senescence is far from elucidated. Methods: In this study, senescent cancer cell models (CaCO2 cells) were constructed by knocking down the ADAR1 gene. RNA immunoprecipitation sequencing was used to identify the o 8 G peaks on messenger RNA (mRNA) of normal CaCO2 cells and senescent CaCO2 cells, and the distribution characteristics of mRNA o 8 G modification were identified. Further bioinformatics analysis of the sequencing data was performed to preliminarily elucidate the potential function of the o 8 G-modified mRNA. Results: There were significant differences in mRNA o 8 G modification distribution between normal and senescent CaCO2 cells. It is suggested that o 8 G modification may play a key role in inducing cancer cells or promoting cancer cell senescence. Gene ontology (GO) enrichment analysis showed that the mRNAs modified by o 8 G were enriched in Cellular component organization or biogenesis, Focal adhesion, and RNA binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the genes modified by o 8 G are concentrated in Focal adhesion signaling pathway, Small cell lung cancer signaling pathway and Proteoglycans in cancer signaling pathway. Conclusion: This study preliminarily revealed the different distribution patterns of o 8 G modification between normal CaCO2 cells and senescent CaCO2 cells. Our study established the link between o 8 G modification and cancer cell senescence, which provides a new insight into the mechanism of cancer cell senescence and a potential therapeutic target for subsequent cancer treatment.
Keywords: Keyword：8-oxoguanine, senescence, RNA methylation, MeRIP-seq, cancer cell
Received: 04 Jun 2024; Accepted: 30 Jan 2025.
Copyright: © 2025 Huang, Lin, Zhao and Wei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jingwen Huang, Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao, China
Yu Lin, Nanfang Hospital, Southern Medical University, Guangzhou, China
Yingying Zhao, Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao, China
Lingbo Wei, Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao, China
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