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ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Cancer Cell Biology
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1510264
This article is part of the Research Topic Organoids as Advanced Model in Cancer Biology: The Drug Screening and Clinical Applications View all articles

Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis

Provisionally accepted
Jingyao Chen Jingyao Chen Shumin Cheng Shumin Cheng Liang Gu Liang Gu Zhangsen Huang Zhangsen Huang Changhua Zhang Changhua Zhang Chunhui Sun Chunhui Sun Songyao Chen Songyao Chen *
  • Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China

The final, formatted version of the article will be published soon.

    Background: Sigmoid colon cancer with spinal metastasis is rare in distant metastasis. In addition, prognosis of colon cancer patients with spinal metastasis was extremely poor. In order to find effective therapy agents, we need to know the biological characteristics of such patients from related models. Methods: We collected sigmoid colon cancer tissue from a young female who has been diagnosed sigmoid colon cancer with multiple spinal metastasis. We successfully established a sigmoid colon cancer organoid investigated the drug screening in a young female sigmoid colon cancer patient with spinal metastasis. HE staining, immunohistochemistry, DNA-sequencing were utilized to compare the biological characteristics between original tumor and organoid. Furthermore, we investigated the drug screening of the sigmoid colon cancer organoid in vitro. Results: A colon cancer organoid from sigmoid colon cancer with spinal metastasis was successfully established. The organoid culture maintained the morphological, histological features, and genomic landscape of the corresponding sigmoid colon cancer cells. Moreover, we performed drug screening test to evaluate the effects of chemotherapeutic drugs and targeted drugs. Conclusions: The sigmoid colon cancer organoid with spinal metastasis was a favorable preclinical model to explore the clinical-pathological characteristics of colon cancer patients with spinal metastasis.

    Keywords: Sigmoid colon cancer, Spinal metastasis, Genomic features, organoid culture, drug screening

    Received: 12 Oct 2024; Accepted: 29 Nov 2024.

    Copyright: © 2024 Chen, Cheng, Gu, Huang, Zhang, Sun and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Songyao Chen, Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China

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