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ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Membrane Traffic and Organelle Dynamics
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1509123
This article is part of the Research Topic Plant and Probiotic Nanovesicles: Innovations and Applications View all articles

Citri Reticulate Pericranium -derived extracellular vesicles exert antioxidant and anti-inflammatory properties and enhance the bioactivity of nobiletin by forming EVs-nob nanoparticles

Provisionally accepted
Ling Ma Ling Ma 1Zimao Ye Zimao Ye 2Dongqin Guo Dongqin Guo 1Chao Nie Chao Nie 2Zhiqin Zhou Zhiqin Zhou 2,3*
  • 1 Chongqing Three Gorges Medical College, Chongqing, China
  • 2 Southwest University, Chongqing, China
  • 3 The Southwest Institute of Fruits Nutrition, chongqing, China

The final, formatted version of the article will be published soon.

    Plant-driven extracellular vesicles (PEVs) have attracted significant interest due to their natural origin, remarkable bioactivity, and efficacy in drug encapsulation and target delivery. In our work, extracellular vesicles from Citri Reticulate Pericranium (CEVs) were isolated and investigated their physicochemical characteristics and biological activities. We identified the vesicle structures as regular, with a particle size of approximately 200 nm. We also detected large quantities of lipids, proteins, carbohydrates, as well as total phenols and total flavonoids. The rich content of CEVs endows them with significant antioxidant and anti-inflammatory effects, which can notably increase the levels of GSH and effectively promote the activity of antioxidant markers such as SOD, CAT, and GR. Additionally, they can inhibit the level of inflammatory markers like NO and inflammatory biological factors(IL-6, IL-1β and TNF-α). In addition, we successfully synthesized EVs-nob nanoparticles with a 83.75±2.83% encapsulation rate and 2.79±0.02% drug loading, which may enhance the antioxidant and anti-inflammatory effects of nobiletin. Our research provides critical insights into the bioactivity of CEVs and demonstrates the significant potential of PEVs in nanocarrier creation, thereby promoting the advancement of more PEVs for biomedical applications.

    Keywords: Citri Reticulate Pericranium, extracellular vesicles, antioxidant, anti-inflammatory, Nobiletin

    Received: 10 Oct 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 Ma, Ye, Guo, Nie and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zhiqin Zhou, Southwest University, Chongqing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.