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ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Stem Cell Research
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1502332
This article is part of the Research Topic Muscle Stem Cell for Skeletal Muscle Regeneration View all articles

Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix

Provisionally accepted
Kitora Dohi Kitora Dohi Yasuko Manabe Yasuko Manabe Nobuharu L Fujii Nobuharu L Fujii Yasuro Furuichi Yasuro Furuichi *
  • Tokyo Metropolitan University, Hachioji, Japan

The final, formatted version of the article will be published soon.

    Cell therapy of skeletal muscles is a promising approach for the prevention of muscular diseases and age-related muscle atrophy. However, cell transplantation to treat muscle atrophy that does not involve disease, such as sarcopenia, is considered impossible because externally injected cells rarely engraft into non-injured muscle tissue. Additionally, skeletal muscle-specific somatic stem cells, known as satellite cells, lose their ability to adhere to tissues after being cultured in vitro and to differentiate to myoblasts. To overcome these hurdles, we explored the use of extracellular matrix (ECM) components to create a niche environment conducive for myoblasts during transplantation. We successfully demonstrated that myoblasts mixed with ECM components can be engrafted into intact skeletal muscle and can significantly increase muscle mass in a mouse model. These findings implicate cell transplantation therapy as a viable option for the treatment of sarcopenia. The findings will inform advancements in regenerative medicine for skeletal muscles.

    Keywords: skeletal muscle, Myoblast, muscle stem cell, Cell Transplantation, Extracellular Matrix

    Received: 26 Sep 2024; Accepted: 29 Nov 2024.

    Copyright: © 2024 Dohi, Manabe, Fujii and Furuichi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yasuro Furuichi, Tokyo Metropolitan University, Hachioji, Japan

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.