AUTHOR=Kazama Kousuke , Miyagoshi Yuki , Nishizono Hirofumi 

TITLE=Tle6 deficiency in male mice led to abnormal sperm morphology and reduced sperm motility

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=12

YEAR=2024

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1481659

DOI=10.3389/fcell.2024.1481659

ISSN=2296-634X

ABSTRACT=<p>Infertility affects over 15% of the global population, and genetic mutations are a substantial cause of infertility. Recent studies have focused on the subcortical maternal complex and its role in early embryonic development. TLE6, a core protein in the subcortical maternal complex, is crucial for female fertility; however, its role in male germ cells remains poorly understood. In this study, we generated a novel <italic>Tle6</italic> knockout mouse model using CRISPR-Cas9 to examine the impact of <italic>Tle6</italic> mutations on male fertility. <italic>Tle6</italic> knockout males exhibited a reduced total sperm count compared to wild-type mice, with a marked decrease in highly motile sperm. Histological observation of <italic>Tle6</italic><sup>+/−</sup> mouse testes showed no apparent structural changes, though impaired sperm maturation was observed. Immunofluorescence staining showed that TLE6 localizes to the midpiece of sperm. It was also confirmed that the expression of <italic>Tle6</italic> is reduced in <italic>Tle6</italic><sup>+/−</sup> male mice. In addition, <italic>Tle6</italic><sup>+/−</sup> mice exhibited a significant increase in serum testosterone levels compared to wild-type mice. Changes in the expression of genes related to sperm function were also observed in the testes of <italic>Tle6</italic> knockout mice. These findings suggest that TLE6 is involved in sperm production and function, and that mutations in TLE6 may impair the production of functional sperm in humans, potentially leading to infertility.</p>