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ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Cellular Biochemistry
Volume 12 - 2024 |
doi: 10.3389/fcell.2024.1472575
This article is part of the Research Topic Progress in the Application of Biomaterials and Nanotechnology in Cell Biology View all articles
Mechanical Force Promotes Tissue and Molecular Changes in Adipose Tissue Regeneration Post-transplantation
Provisionally accepted- Guangdong Province Women and Children Hospital, Guangzhou, China
Introduction: Fat grafting often yields inconsistent and suboptimal results, necessitating improved fat processing techniques. A stromal vascular fraction (SVF) gel created using mechanical emulsification demonstrates superior retention rates to conventional Coleman fat grafts.This study investigated the mechanisms at play by transplanting fat aspirates from liposuction patients-either processed as Coleman fat grafts or further refined into an SVF gel via mechanical shear force-onto the backs of nude mice.The retention rate of the SVF gel after transplantation surpassed that observed for Coleman fat. Hematoxylin and eosin (HE) staining and immunofluorescence results demonstrated that the SVF gel group could form new adipose tissue characterized by well-organized mature fat structures. Mechanical shear force application induced increased mesenchymal stem cell abundance. Rather than merely surviving regeneration, fat was regenerated after transplantation, and the regenerated cells were mainly from mice, which was supported by microarray analysis. RNA-seq highlighted 601 genes expressed between SVF gel and Coleman fat groups, with 164 genes upregulated (cell cycle processes), and 437 genes downregulated (lipid metabolism).Discussion: The application of mechanical shear force reduces the risk of complications and fosters cell proliferation and division, thereby enhancing the retention and regeneration of transplanted fat.
Keywords: mechanical shear force, Stromal vascular fraction gel, Coleman fat, Tissue Regeneration, Transcriptome
Received: 29 Jul 2024; Accepted: 30 Aug 2024.
Copyright: © 2024 Ye, Ma, Guo, Li, Hu, Tan and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yuan Ye, Guangdong Province Women and Children Hospital, Guangzhou, China
Bingyang Guo, Guangdong Province Women and Children Hospital, Guangzhou, China
Xiongjie Li, Guangdong Province Women and Children Hospital, Guangzhou, China
Kuikui Hu, Guangdong Province Women and Children Hospital, Guangzhou, China
Meijun Tan, Guangdong Province Women and Children Hospital, Guangzhou, China
Liang Zhang, Guangdong Province Women and Children Hospital, Guangzhou, China
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