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ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Epigenomics and Epigenetics
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1472338

Multi-omics study on the effect of moderate intensity exercise on protein of lactylation in mouse muscle tissue

Provisionally accepted
Jiahui Chang Jiahui Chang 1,2,3Wanyu Wu Wanyu Wu 1,2,3Ping Qian Ping Qian 4Zhaoxu Lu Zhaoxu Lu 2Xuejia He Xuejia He 5Fang Wang Fang Wang 2Ting Zhang Ting Zhang 2,3*
  • 1 Children’s Hospital Capital Institute of Pediatrics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
  • 2 Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, Beijing Municipality, China
  • 3 Peking Union Medical College Graduate School, Beijing, China
  • 4 Children's Hospital of Capital Institute of Pediatrics, Beijing, Beijing Municipality, China
  • 5 Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics-Peking University Teaching Hospital, Beijing, China

The final, formatted version of the article will be published soon.

    To explore the effects of moderate intensity exercise on protein of lactylation in mouse muscle tissue metabolism. The healthy adult mice running for 6 weeks as exercise model and sedentary mice as control were used to perform transcriptomic, proteomic, lactylation-proteomics, and metabolomics analysis. In addition, correlation analysis between transcriptome and proteome, and proteome and metabolome was conducted as well. In this study, 159 lactate sites of 78 proteins were identified to be differentially regulated by moderate intensity exercise. Enrichment analysis showed that lactylation of proteins Mtatp8, Atp5mg and Atp5po exhibited ATP hydrolysis activity.They are involved in biological processes such as mitochondrial transmembrane transport, and Mtatp8, Atp5mg and Atp5po participate in Oxidative phosphorylation and Thermogenesis pathways. The lactylation levels of Mtatp8, Atp5mg and Atp5po proteins in exercise group were significantly decreased, while their protein levels were significantly increased. The combined analysis of proteomics and metabolomics showed that the Oxocarboxylic acid metabolism pathway and Sphingolipid signaling pathway had significant changes under the influence of moderate intensity exercise. Our results showed that moderate intensity exercise has a certain effect on the lactylation level of mice, possibly by reducing lactylation levels of Mtatp8, Atp5mg and Atp5po and increasing the expression of their protein levels, thereby regulating the Oxidative phosphorylation pathway and participating in energy metabolism. 2-Oxocarboxylic acid metabolism pathway and Sphingolipid signaling pathway need to be further explored.

    Keywords: Moderate intensity exercise, lactylation, Proteome, Metabolome, Muscle tissue

    Received: 29 Jul 2024; Accepted: 30 Dec 2024.

    Copyright: © 2024 Chang, Wu, Qian, Lu, He, Wang and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ting Zhang, Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, Beijing Municipality, China

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