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ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Cellular Biochemistry
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1469080

Impact of UPF2 on the levels of CD81 on extracellular vesicles

Provisionally accepted
Chaehwan Oh Chaehwan Oh 1Krystyna Mazan-Mamczarz Krystyna Mazan-Mamczarz 2Ji Heon Noh Ji Heon Noh 1Myriam Gorospe Myriam Gorospe 2Kyoung Mi Kim Kyoung Mi Kim 1*
  • 1 Chungnam National University, Daejeon, Republic of Korea
  • 2 National Institute on Aging, Baltimore, United States

The final, formatted version of the article will be published soon.

    Extracellular vesicles (EVs) are involved in cell-to-cell communication. Following uptake, EV cargo molecules, including DNA, RNA, lipids, and proteins, influence gene expression and molecular signaling in recipient cells. Although various studies have identified disease-specific EV molecules, further research into their biogenesis and secretion mechanisms is needed for clinical application. Here, we investigated the role of UPF2 in regulating the biogenesis and components of EVs. Notably, UPF2 promoted the expression of CD81, a membrane protein marker of EVs, as UPF2 knockdown decreased CD81 levels in EVs, both inside the cell and secreted. In contrast, the expression levels of CD63 increased, without altering the size or numbers of EVs. In addition, reducing UPF2 did not affect the total number of EVs but lowered production of CD81-positive EVs and reduced the efficiency of uptake by recipient cells.Collectively, our findings uncover a novel function for UPF2 in regulating the production of CD81 and EV properties.

    Keywords: UPF2, CD81, extracellular vesicles, EV biogenesis, EV uptake, exosome

    Received: 23 Jul 2024; Accepted: 29 Oct 2024.

    Copyright: © 2024 Oh, Mazan-Mamczarz, Noh, Gorospe and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Kyoung Mi Kim, Chungnam National University, Daejeon, Republic of Korea

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.