AUTHOR=Jiménez-Loygorri Juan Ignacio , Jiménez-García Carlos , Viedma-Poyatos Álvaro , Boya Patricia TITLE=Fast and quantitative mitophagy assessment by flow cytometry using the mito-QC reporter JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=12 YEAR=2024 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1460061 DOI=10.3389/fcell.2024.1460061 ISSN=2296-634X ABSTRACT=
Mitochondrial quality control is finely tuned by mitophagy, the selective degradation of mitochondria through autophagy, and mitochondrial biogenesis. Removal of damaged mitochondria is essential to preserve cellular bioenergetics and prevent detrimental events such as sustained mitoROS production, pro-apoptotic cytochrome c release or mtDNA leakage. The array of tools available to study mitophagy is very limited but in constant development. Almost a decade ago, we developed a method to assess mitophagy flux using MitoTracker Deep Red in combination with lysosomal inhibitors. Now, using the novel tandem-fluorescence reporter