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ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Stem Cell Research
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1450543
This article is part of the Research Topic Current Methods for Detecting and Diagnosing Stem Cell Pathogenesis: A Focus on Translational Interventions in the Contemporary Disease and Technology Landscape View all 7 articles

Differentiation ability of Hematopoietic stem cells and Mesenchymal stem cells isolated from Human peripheral blood

Provisionally accepted
Echambadi Loganathan Samundeshwari Echambadi Loganathan Samundeshwari Surekha Kattaru Surekha Kattaru Sireesha Kodavala Sireesha Kodavala Chodimella Chandrasekhar Chodimella Chandrasekhar PVGK Sarma PVGK Sarma *
  • Sri Venkateswara Institute of Medical Sciences, Tirupati, India

The final, formatted version of the article will be published soon.

    The Human Hematopoietic stem cells (HSCs) and Mesenchymal stem cells (MSCs) are the major stem cells of bone marrow and are usually isolated from the peripheral blood. In the present study, we have isolated these stem cells by apheresis method from a donor who was administered with G-CSF. In vitro propagation of these stem cells shows plastic adherence property expressing CD73 and CD105 surface markers a characteristic feature of MSCs.Non-adherent cells growing as suspension culture are the HSCs, expressing CD150, CD133, CD34, and CD45 on their surface which regulate the quiescence nature and they derive energy from anaerobic glycolysis. The HSCs grow slowly compared to MSCs and are more viable, and survive for long periods under in vitro conditions which are due to the expression of telomerase, BCL2, and Notch genes. The poor viability of MSCs in culture due to prominent expression of apoptotic genes BAX, caspase 3, caspase 9 leading rapid apoptosis. This was evident even in cells (astrocytes, osteocytes, and beta cells of islets of Langerhans) differentiated from HSCs and MSCs. Highlighting the importance of HSCs, the naive stem cells, in regeneration of tissues.

    Keywords: Pheripheral blood stem cells, HSCs, MSCs, differentiation ability, Regenerative Medicine

    Received: 17 Jun 2024; Accepted: 21 Oct 2024.

    Copyright: © 2024 Samundeshwari, Kattaru, Kodavala, Chandrasekhar and Sarma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: PVGK Sarma, Sri Venkateswara Institute of Medical Sciences, Tirupati, India

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