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ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Molecular and Cellular Pathology
Volume 12 - 2024 |
doi: 10.3389/fcell.2024.1450215
IMPC-based screening revealed that ROBO1 can regulate osteoporosis by inhibiting osteogenic differentiation
Provisionally accepted
Xiangzheng Zhang
1*
Yike Wang
1
Miao Zheng
1*
Qi Wei
1*
Ruizhi Zhang
1*
Keyu Zhu
1*
Qiaocheng Zhai
2*
Youjia Xu
1*- 1 Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
- 2 the Quzhou Affiliated Hospital of Wenzhou Medical University,, Quzhou, China
The utilization of denosumab in treating osteoporosis highlights promising prospects for osteoporosis intervention guided by gene targets. While omics-based research into osteoporosis pathogenesis yields a plethora of potential gene targets for clinical transformation, identifying effective gene targets has posed challenges. This study addresses this issue by integrating clinical osteoporosisrelated omics data and leveraging the International Mouse Phenotyping Consortium (IMPC) database. Through this approach, we've developed a method for accurately identifying gene targets capable of directly regulating osteogenic differentiation. We also verified the function of ROBO1 in Saos-2, 3T3-E1 and bone marrow mesenchymal stem cells (BMSCs), and found that the weakened osteogenic differentiation function in 3T3-E1 caused by downregulation of Robo1 may be related to the activation of inflammation signaling pathways. Our study provides a new regulatory molecular target for the pathogenesis of osteoporosis, which is expected to provide new solutions for the osteoporosis intervention.
Keywords: Osteoporosis, IMPC, ROBO1, Osteogenesis, Inflammation
Received: 10 Jul 2024; Accepted: 26 Sep 2024.
Copyright: © 2024 Zhang, Wang, Zheng, Wei, Zhang, Zhu, Zhai and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xiangzheng Zhang, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
Miao Zheng, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
Qi Wei, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
Ruizhi Zhang, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
Keyu Zhu, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
Qiaocheng Zhai, the Quzhou Affiliated Hospital of Wenzhou Medical University,, Quzhou, China
Youjia Xu, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China
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