AUTHOR=Tago Tatsuya , Yamada Yumi , Goto Yumi , Toyooka Kiminori , Ochi Yuka , Satoh Takunori , Satoh Akiko K. 

TITLE=Golgi clustering by the deficiency of COPI-SNARE in Drosophila photoreceptors

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=12

YEAR=2024

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1442198

DOI=10.3389/fcell.2024.1442198

ISSN=2296-634X

ABSTRACT=<p>A comprehensive study of soluble <italic>N</italic>-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) in the fly genome by RNAi in <italic>Drosophila</italic> photoreceptors indicated that knockdown of any of the COPI-SNAREs, <italic>Syx18</italic>, <italic>Sec20</italic>, and <italic>Use1</italic>, resulted in the same characteristic phenotypes: Golgi stacks gathering on their <italic>trans</italic>-side, laterally expanded Golgi cisternae, and a reduced number of discrete Golgi stacks. These Golgi stacks are reminiscent of mammalian Golgi ribbons and Brefeldin A (BFA)-bodies in <italic>Drosophila</italic> S2 cells. As previously reported, BFA suppresses <italic>trans</italic>-Golgi network (TGN) fission and Golgi stack separation to form a BFA-body, which is a cluster of Golgi stacks cored by recycling endosomes. We found that the impairing each of COPI-SNAREs results in clustered Golgi stacks similar to BFA-bodies, indicating that COPI-SNAREs have a role to separate clustered Golgi stacks. These results further support the idea that the movement of Golgi stacks and the balance of fusion and fission of the TGN determine the level of clustering and ribbon formation of Golgi stacks within cells.</p>