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REVIEW article

Front. Cell Dev. Biol.
Sec. Stem Cell Research
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1435461

Navigating Stem Cell Culture: Insights, Techniques, Challenges, and Prospects

Provisionally accepted
Aleksandra Górska Aleksandra Górska 1Mateusz Trubalski Mateusz Trubalski 1Bartosz Borowski Bartosz Borowski 1Adam Brachet Adam Brachet 2Renata Markiewicz Renata Markiewicz 1,3*
  • 1 Medical University of Lublin, Lublin, Poland
  • 2 Department of Forensic Medicine, Medical University of Lublin, Lublin, Poland
  • 3 Occupational Therapy Laboratory, Medical University of Lublin, Lublin, Poland

The final, formatted version of the article will be published soon.

    Stem cell research holds huge promise for regenerative medicine and disease modeling, making the understanding and optimization of stem cell culture a critical aspect of advancing these therapeutic applications. This comprehensive review provides an in-depth overview of stem cell culture, including general information, contemporary techniques, encountered problems, and future perspectives. The article begins by explaining the fundamental characteristics of various stem cell types, elucidating the importance of proper culture conditions in maintaining pluripotency or lineage commitment. A detailed exploration of established culture techniques sheds light on the evolving landscape of stem cell culture methodologies. Common challenges such as genetic stability, heterogeneity, and differentiation efficiency are thoroughly discussed, with insights into cutting-edge strategies and technologies aimed at addressing these hurdles. Moreover, the article delves into the impact of substrate materials, culture media components, and biophysical cues on stem cell behavior, emphasizing the intricate interplay between the microenvironment and cell fate decisions. As stem cell research advances, ethical considerations and regulatory frameworks become increasingly important, prompting a critical examination of these aspects in the context of culture practices. Lastly, the article explores emerging perspectives, including the integration of artificial intelligence and machine learning in optimizing culture conditions, and the potential applications of stem cell-derived products in personalized medicine. This comprehensive overview aims to serve as a valuable resource for researchers and clinicians, fostering a deeper understanding of stem cell culture and its key role in advancing regenerative medicine and biomedical research.

    Keywords: stem, Cells, culture, methods, pluripotency, Perspectives

    Received: 03 Jun 2024; Accepted: 09 Oct 2024.

    Copyright: © 2024 Górska, Trubalski, Borowski, Brachet and Markiewicz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Renata Markiewicz, Medical University of Lublin, Lublin, Poland

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.