Skip to main content

ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Cell Death and Survival
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1416345
This article is part of the Research Topic Ferroptosis: Intersections, Implications, and Innovations in Programmed Cell Death View all 5 articles

Identification and validation of Ferroptosis-Related biomarkers in Intervertebral Disc Degeneration

Provisionally accepted
Chenglong Li Chenglong Li 1Chengshuo Fei Chengshuo Fei 1Shiyong Le Shiyong Le 2Zhongming Lai Zhongming Lai 1Bo Yan Bo Yan 2Liang Wang Liang Wang 2*Zhongmin Zhang Zhongmin Zhang 1*
  • 1 Nanfang Hospital, Southern Medical University, Guangzhou, China
  • 2 Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong Province, China

The final, formatted version of the article will be published soon.

    Ferroptosis plays an important role in intervertebral disc degeneration (IDD). We aim to identify key genes that regulate ferroptosis within IDD to uncover the fundamental mechanisms of the disease. we analyzed public datasets (GSE23130 and GSE70362) and the FerrDb database to identify ferroptosisrelated genes (DE-FRGs) involved in IDD, including MT1G, CA9, AKR1C1, AKR1C2, DUSP1, CIRBP, and KLHL24. Validation with single-cell RNA sequencing (GSE199866) confirmed the specific roles and expression patterns of these genes, particularly spotlighting the roles of MT1G and CA9. Subsequently, we used immunohistochemistry and Western blot analyses in both clinical and mouse models, the protein expression levels of these DE-FRGs in various tissues were identified. Additionally, we explored how these genes influence immune cell infiltration in the IDD microenvironment, highlighting potential immune-modulatory roles. Conclusively, our study elucidates the crucial role of ferroptosis in IDD and identifies specific genes as potential targets for diagnosis and therapy.

    Keywords: Intervertebral Disc Degeneration, ferroptosis, Bioinformatics analysis, Immune Cell Infiltration, single-cell RNA sequencing

    Received: 12 Apr 2024; Accepted: 26 Aug 2024.

    Copyright: © 2024 Li, Fei, Le, Lai, Yan, Wang and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Liang Wang, Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong Province, China
    Zhongmin Zhang, Nanfang Hospital, Southern Medical University, Guangzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.