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ORIGINAL RESEARCH article

Front. Cell Dev. Biol.
Sec. Cancer Cell Biology
Volume 12 - 2024 | doi: 10.3389/fcell.2024.1410308
This article is part of the Research Topic LncRNAs In Tumor Microenvironment: Its Role in Immunoregulation and Inflammation View all 4 articles

Long Noncoding RNA, PURPL is associated with aneuploidy and its magnitude of expression level is dependent on P53 status

Provisionally accepted
Pushkar Malakar Pushkar Malakar *
  • Ramakrishna Mission Vivekananda Educational and Research Institute, Howrah, India

The final, formatted version of the article will be published soon.

    Long non-coding RNAs (lncRNAs) are a fascinating, but still largely uncharacterized, class of genes. Recently, lncRNAs have attracted significant attention due to their emerging functions in development and disease. The role of lncRNAs in chromosome instability or aneuploidy is not extensively studied. We started with the objective of characterizing lncRNAs that play an important role in chromosome instability (CIN) or aneuploidy. Studies have demonstrated that CIN, or aneuploidy, controls the tumor microenvironment and stimulates inflammatory signaling. Here, we report the initial functional characterization of PURPL in the context of chromosomal instability or aneuploidy. We report the expression of lncRNA PURPL in three experimental models of chromosomal instability, or aneuploidy. In addition, the study also showed that the extent or magnitude of PURPL expression is dependent upon p53 status. Our research also showed that turning off PURPL is enough to create a CIN phenotype in RPE-1 cell lines that were previously karyotypically stable. Moreover, PURPL knockdown cells are more sensitive to CIN or aneuploidy inducers. This shows PURPL is essential for maintaining chromosomal or genomic stability in mammalian cells. Collectively, the study demonstrated that lncRNA-PURPL significantly contributes to CIN, or aneuploidy.

    Keywords: long noncoding RNA, PURPL, Chromosomal Instability, p53, multinucleation, Altered Nuclear Shape, and Aneuploidy

    Received: 31 Mar 2024; Accepted: 27 Aug 2024.

    Copyright: © 2024 Malakar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Pushkar Malakar, Ramakrishna Mission Vivekananda Educational and Research Institute, Howrah, India

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