AUTHOR=Lake Katherine E. , Colonnetta Megan M. , Smith Clayton A. , Saunders Kaitlyn , Martinez-Algarin Kenneth , Mohta Sakshi , Pena Jacob , McArthur Heather L. , Reddy Sangeetha M. , Roussos Torres Evanthia T. , Chen Elizabeth H. , Chan Isaac S. 

TITLE=Digital droplet PCR analysis of organoids generated from mouse mammary tumors demonstrates proof-of-concept capture of tumor heterogeneity

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=12

YEAR=2024

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1358583

DOI=10.3389/fcell.2024.1358583

ISSN=2296-634X

ABSTRACT=<p>Breast cancer metastases exhibit many different genetic alterations, including copy number amplifications (CNA). CNA are genetic alterations that are increasingly becoming relevant to breast oncology clinical practice. Here we identify CNA in metastatic breast tumor samples using publicly available datasets and characterize their expression and function using a metastatic mouse model of breast cancer. Our findings demonstrate that our organoid generation can be implemented to study clinically relevant features that reflect the genetic heterogeneity of individual tumors.</p>