AUTHOR=Sofi Sajad , Coverley Dawn 

TITLE=CIZ1 in Xist seeded assemblies at the inactive X chromosome

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1296600

DOI=10.3389/fcell.2023.1296600

ISSN=2296-634X

ABSTRACT=<p>There is growing evidence that X-chromosome inactivation is driven by phase-separated supramolecular assemblies. However, among the many proteins recruited to the inactive X chromosome by <italic>Xist</italic> long non-coding RNA, so far only a minority (CIZ1, CELF1, SPEN, TDP-43, MATR3, PTBP1, PCGF5) have been shown to form <italic>Xist</italic>-seeded protein assemblies, and of these most have not been analyzed in detail. With focus on CIZ1, here we describe 1) the contribution of intrinsically disordered regions in RNA-dependent protein assembly formation at the inactive X chromosome, and 2) enrichment, distribution, and function of proteins within <italic>Xist</italic>-seeded assemblies.</p>