Corrigendum: Reconstitution of phase-separated signaling clusters and actin polymerization on supported lipid bilayers

A Corrigendum on
Reconstitution of phase-separated signaling clusters and actin polymerization on supported lipid bilayers

by Cheng X, Ullo MF and Case LB (2022). Front. Cell Dev. Biol. 10:932483. doi: 10.3389/fcell.2022.932483

In the published article, there was an error in the captions for Figures 4–6 as published. In these captions, “0.25 µM” should be “250 µM.” The corrected captions appears below.

“FIGURE 4 | Actin polymerization from phase separated clusters. (A) Total Internal Reflection Fluorescence (TIRF) imaging of 1 µM actin (5% labeled by Alexa 647, magenta) polymerization with 3 nM Arp2/3, 250 µM ATP, 0.1 mM MgCl₂ and 0.1 mM EGTA, from clusters made from 500 nM Nck (15% labeled by Alexa488, green), 250 nM N-WASP and 2.5 nM His₈-nephrin on the membrane. (B) Zoom-in view of region indicated by white box in (A). Scale bars, 10 µm. Total Internal Reflection Fluorescence (TIRF) penetration depth 200 nm for all channels.”

“FIGURE 5 | Actin polymerization with different levels of capping proteins. (A) 1 µM actin (5% labeled by Alexa647) polymerization is induced by 3 nM Arp2/3, 250 µM ATP, 0.1 mM MgCl₂ and 0.1 mM EGTA, with different amounts of CapZ included in the system (indicated above each image), from clusters made from 500 nM Nck, 250 nM N-WASP and 1.25 nM His₈-nephrin (15% labeled by Alexa405) on the membrane. Individual images are TIRF image overlays of nephrin-405 shown in cyan and actin-647 shown in magenta. Total Internal Reflection Fluorescence (TIRF) penetration depth 200 nm for all channels. Scale bar, 5 µm. (B) Quantification of actin mean and fraction intensity over time for the conditions showed in (A). Actin was selected by selecting the intensity threshold between 250 and 65535 the individual 16-bit images, values for each frame are calculated as described in Section 4.4.”

“FIGURE 6 | Representative actin polymerization analysis (A) 1 µM actin (5% labeled by Alexa647) polymerization is induced by 3 nM Arp2/3, 6 nM CapZ, 250 µM ATP, 0.1 mM MgCl₂ and 0.1 mM EGTA, from clusters made from 1 µM Nck, 2 μM N-WASP (15% Alexa488) and 1.25 nM His₈-nephrin on the membrane. Binary mask from segmentation of the N-WASP images. Pixels outside the clusters are white, pixels inside the clusters are black. Scale bar, 5 µm. (B) Quantification of actin polymerization over time for the condition showed in (A). Graph was generated in Matlab. Black dotted lines indicate a linear fit of the five points around the half-max intensity.”

The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

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