AUTHOR=Ide Hayato , Hayashida Yukihisa , Morimoto Yusuke V. 

TITLE=Visualization of c-di-GMP in multicellular Dictyostelium stages

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1237778

DOI=10.3389/fcell.2023.1237778

ISSN=2296-634X

ABSTRACT=<p>The bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) is only synthesized and utilized by the cellular slime mold <italic>Dictyostelium discoideum</italic> among eukaryotes. <italic>Dictyostelium</italic> cells undergo a transition from a unicellular to a multicellular state, ultimately forming a stalk and spores. While <italic>Dictyostelium</italic> is known to employ c-di-GMP to induce differentiation into stalk cells, there have been no reports of direct observation of c-di-GMP using fluorescent probes. In this study, we used a fluorescent probe used in bacteria to visualize its localization within <italic>Dictyostelium</italic> multicellular bodies. Cytosolic c-di-GMP concentrations were significantly higher at the tip of the multicellular body during stalk formation.</p>