AUTHOR=Yi Mingliang , Wang Mingyue , Xu Yongjie , Cao Zhikun , Ling Yinghui , Zhang Zijun , Cao Hongguo 

TITLE=CRISPR-based m6A modification and its potential applications in telomerase regulation

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1200734

DOI=10.3389/fcell.2023.1200734

ISSN=2296-634X

ABSTRACT=<p>Telomerase determines cell lifespan by controlling chromosome stability and cell viability, m<sup>6</sup>A epigenetic modification plays an important role in the regulation of telomerase activity. Using CRISPR epigenome editing to analyze specific m<sup>6</sup>A modification sites in telomerase will provide an important tool for analyzing the molecular mechanism of m<sup>6</sup>A modification regulating telomerase activity. In this review, we clarified the relevant applications of CRISPR system, paid special attention to the regulation of m<sup>6</sup>A modification in stem cells and cancer cells based on CRISPR system, emphasized the regulation of m<sup>6</sup>A modification on telomerase activity, pointed out that m<sup>6</sup>A modification sites regulate telomerase activity, and discussed strategies based on telomerase activity and disease treatment, which are helpful to promote the research of anti-aging and tumor related diseases.</p>