AUTHOR=Jiang Shan , Zheng Jianfeng , Cui Zhaolei , Li Yanhong , Wu Qiaoling , Cai Xintong , Zheng Chaoqiang , Sun Yang 

TITLE=FBXO5 acts as a novel prognostic biomarker for patients with cervical cancer

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1200197

DOI=10.3389/fcell.2023.1200197

ISSN=2296-634X

ABSTRACT=<p><bold>Background:</bold> Cervical cancer (CC) remains one of the most common and deadly malignancies in women worldwide. <italic>FBXO5</italic>, a protein-coding gene, is highly expressed in a variety of primary tumors and promotes tumor progression, however, its role and prognostic value in CC remain largely unknown.</p><p><bold>Methods:</bold> A key differential gene, <italic>FBXO5</italic>, was screened according to WGCNA based on immunohistochemical assays of clinical samples, multiple analyses of the Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases, including survival analysis, tumor mutational burden, GO, KEGG, tumor immune infiltration, and chemotherapeutic drug sensitivity, to explore the expression and prognostic value of <italic>FBXO5</italic> in CC. The migration and invasiveness of cervical cancer cells following <italic>FBXO5</italic> knockdown and overexpression were examined using wound healing and transwell assays, and the viability of cancer cells was assessed using CCK8 and EdU assays.</p><p><bold>Results:</bold><italic>FBXO5</italic> was discovered to be substantially expressed in CC tissues using data from our CC cohort and the TCGA database, and a survival analysis indicated <italic>FBXO5</italic> as a predictive factor for poor overall survival in CC patients. <italic>In vitro</italic>, CC cells were more inclined to proliferate, migrate, and invade when <italic>FBXO5</italic> was upregulated as opposed to when it was knocked down.</p>