AUTHOR=Puvogel Sofía , Blanchard Kris , Casas Bárbara S. , Miller Robyn L. , Garrido-Jara Delia , Arizabalos Sebastián , Rehen Stevens K. , Sanhueza Magdalena , Palma Verónica TITLE=Altered resting-state functional connectivity in hiPSCs-derived neuronal networks from schizophrenia patients JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.935360 DOI=10.3389/fcell.2022.935360 ISSN=2296-634X ABSTRACT=
Schizophrenia (SZ) is a severe mental disorder that arises from abnormal neurodevelopment, caused by genetic and environmental factors. SZ often involves distortions in reality perception and it is widely associated with alterations in brain connectivity. In the present work, we used Human Induced Pluripotent Stem Cells (hiPSCs)-derived neuronal cultures to study neural communicational dynamics during early development in SZ. We conducted gene and protein expression profiling, calcium imaging recordings, and applied a mathematical model to quantify the dynamism of functional connectivity (FC) in hiPSCs-derived neuronal networks. Along the neurodifferentiation process, SZ networks displayed altered gene expression of the glutamate receptor-related proteins