AUTHOR=Panara Virginia , Monteiro Rui , Koltowska Katarzyna 

TITLE=Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development—New Insights From Technical Advances

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.891538

DOI=10.3389/fcell.2022.891538

ISSN=2296-634X

ABSTRACT=<p>Epigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish represents a powerful model to investigate the activity of <italic>cis</italic>-regulatory elements <italic>in vivo</italic> by combining technologies such as ATAC-seq, ChIP-seq and CUT&amp;Tag with the generation of transgenic lines and live imaging to validate the activity of these regulatory elements. Recently, this approach led to the identification and characterization of key enhancers of important vascular genes, such as <italic>gata2a, notch1b</italic> and <italic>dll4</italic>. In this review we will discuss how the latest technologies in epigenetics are being used in the zebrafish to determine chromatin states and assess the function of the <italic>cis</italic>-regulatory sequences that shape the zebrafish vascular network.</p>