AUTHOR=Alves-Paiva Raquel M. , Nascimento Sabrina do , De Oliveira Denise , Coa Larissa , Alvarez Kelen , Hamerschlak Nelson , Okamoto Oswaldo Keith , Marti Luciana C. , Kondo Andrea T. , Kutner Jose Mauro , Bortolini Maria Augusta Tezelli , Castro Rodrigo , Godoy Juliana A. Preto de 

TITLE=Senescence State in Mesenchymal Stem Cells at Low Passages: Implications in Clinical Use

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.858996

DOI=10.3389/fcell.2022.858996

ISSN=2296-634X

ABSTRACT=<p>Mesenchymal stem cells (MSCs) are multipotent cells found in various tissues and are easily cultivated. For use in clinical protocols, MSCs must be expanded to obtain an adequate number of cells, but a senescence state may be instituted after some passages, reducing their replicative potential. In this study, we report a case where MSC derived from an elderly donor acquired a senescence state after three passages. The bone marrow was aspirated from a female patient submitted to a cell therapy for the incontinency urinary protocol; MSCs were cultivated with DMEM low glucose, supplemented with 10% autologous serum (AS) plus 1% L-glutamine and 1% antibiotic/antimycotic. Senescence analysis was performed by β-galactosidase staining after 24 and 48 h. Controls were established using BM-MSC from healthy donors and used for senescence and gene expression assays. Gene expression was performed using RT-PCR for pluripotency genes, such as <italic>SOX2</italic>, <italic>POU5F1</italic>, <italic>NANOG</italic>, and <italic>KLF4.</italic> MSC telomere length was measured by the Southern blotting technique, and MSCs were also analyzed for their capacity to differentiate into adipocytes, chondrocytes, and osteocytes. The patient’s MSC expansion using AS displayed an early senescence state. In order to understand the role of AS in senescence, MSCs were then submitted to two different culture conditions: 1) with AS or 2) with FBS supplementation. Senescence state was assessed after 24 h, and no statistical differences were observed between the two conditions. However, patients’ cells cultured with AS displayed a higher number of senescence cells than FBS medium after 48 h (<italic>p</italic> = 0.0018). Gene expression was performed in both conditions; increased expression of <italic>KLF4</italic> was observed in the patient’s cells in comparison to healthy controls (<italic>p</italic> = 0.0016); reduced gene expression was observed for <italic>NANOG</italic> (<italic>p</italic> = 0.0016) and <italic>SOX2</italic> (<italic>p</italic> = 0.0014) genes. Telomere length of the patient’s cells was shorter than that of a healthy donor and that of a patient of similar age. Osteocyte differentiation seemed to be more diffuse than that of the healthy donor and that of the patient of similar age. MSCs could enter a senescence state during expansion in early passages and can impact MSC quality for clinical applications, reducing their efficacy when administered.</p>