AUTHOR=Sun Bo , Meng Xianhui , Li Yumin , Li Yanlong , Liu Rui , Xiao Zhongdang 

TITLE=Conditioned medium from human cord blood mesenchymal stem cells attenuates age-related immune dysfunctions

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.1042609

DOI=10.3389/fcell.2022.1042609

ISSN=2296-634X

ABSTRACT=<p>Aging is accompanied with progressive deterioration of immune responses and tissue’s function. Using 12-month-old mice as model, we showed that conditioned medium of human cord blood mesenchymal stem cells (CBMSC-CM) significantly reduced the population percentage of CD3<sup>−</sup>CD335<sup>+</sup> NK and CD4<sup>+</sup>CD25<sup>+</sup> regulatory T-cells in peripheral blood. The CBMSC-CM administration also increased naïve T-cells number and restored the ratio of naïve to memory T-cells in CD4<sup>+</sup> T-cells population. These results indicated that CBMSC-CM improved the immune response efficiency of aged mice. Moreover, we also found CBMSC-CM treatment significantly reduced the number of senescenT-cells in kidney tissues. Finally, we demonstrated that CBMSC-CM remarkably attenuated hydrogen peroxide triggered T-cell response and ameliorated oxidative stress induced cellular senescence. All of these data suggest a prominent anti-aging effect of secretome of CBMSCs.</p>