AUTHOR=Wang Yibo , Wang Wenji , Cheng Kaixin , Geng Kaiying , Liang Jing , Wang Peike , Zhang Jiawei , Niu Shudong , Jia Longzhong , Zhang Shuo , Li Lingyu , Feng Xiean , Wang Chao , Wang Haibin , Zhang Hua , Zhang Yan TITLE=Polycomb subunit Pcgf2 mediates ovulation and fertility through transcriptional regulation progesterone receptor JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.1010601 DOI=10.3389/fcell.2022.1010601 ISSN=2296-634X ABSTRACT=
Ovarian follicles are the fundamental structure to support oocyte development, which provides mature oocytes for offspring. This process requires granulosa cells (GCs) to respond to the midcycle surge of hormones, leading to GC proliferation and differentiation by a series of genes’ transcriptional expression changes. Epigenetic mediator, Polycomb Repressive Complex 1 (PRC1) has been reported to function in fetal ovarian development. However, its functional relevance to folliculogenesis and ovulation remains unknown. In this study, we demonstrated that GC-selective depletion of PCGF2, a key component of PRC1, led to the loss of follicles, ovulation defects, and a lengthened estrus cycle, resulting in subfertility in female mice. The expression of PCGF2 is in the GCs of growing follicles and increases after human chorionic gonadotropin (hCG) stimulation. PCGF2 bound to the promoter of the key ovulation gene progesterone receptor (