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ORIGINAL RESEARCH article

Front. Cell Dev. Biol., 25 November 2021
Sec. Membrane Traffic and Organelle Dynamics
This article is part of the Research Topic Molecular Basis of Membrane Dynamics in Health and Disease View all 8 articles

AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia

Shimin WangShimin Wang1Longfeng YaoLongfeng Yao1Wenjuan ZhangWenjuan Zhang1Zihang ChengZihang Cheng1Can HuCan Hu1Hang LiuHang Liu1Yanling Yan,Yanling Yan1,2Anbing Shi,
Anbing Shi1,2*
  • 1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
  • 2Cell Architecture Research Institute, Huazhong University of Science and Technology, Wuhan, China

Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in C. elegans intestinal epithelia.

Introduction

In the conventional secretion pathway, cargo proteins traverse ER-Golgi and reach the plasma membrane via transport vesicles (Mellman and Warren, 2000; Rabouille, 2017; Dimou and Nickel, 2018). There are apical and basolateral membrane domains in epithelial cells, which leads to additional complexity of cargo sorting (Yeaman et al., 1999; Ang et al., 2003; Sato et al., 2007; Nakajo et al., 2016). Accumulating evidence indicated that the trans-Golgi network (TGN) functions as a sorting organelle during secretion in epithelial cells (Mellman and Warren, 2000; Gravotta et al., 2007; Thuenauer et al., 2014). Apical and basolateral proteins must be separated in TGN before their inclusion into separate routes. To ensure polarized secretion, delicate sorting machinery is employed to package the cargo proteins into specific vesicles and then deliver them to various downstream destinations. TGN missorting can lead to inappropriate targeting of cargo proteins and cell polarity defects (Guo et al., 2014).

Cargo adaptors and clathrin are required for the proper TGN sorting. Once recruited onto TGN, cargo adaptors recognize the sorting motif within the cytoplasmic domain of the transmembrane proteins. Then, clathrin is recruited to TGN and thus facilitates sorting the cargos into the specific membrane carriers (Guo et al., 2014). As a heterotetramer, AP complex contains two large subunits (α, β, γ, δ, ε, or ζ), one medium subunit (μ1–μ5) and one small subunit (σ1–σ5) (Nakatsu et al., 2014). Previous studies have shown that AP-1 is implicated in the cargo sorting at the TGN (Brodsky et al., 2001; Nakatsu et al., 2014). In mammals, two AP-1 adaptor complexes have been identified, including AP-1A and AP-1B (Folsch et al., 2003; Shteyn et al., 2011). C. elegans genome encodes five AP-1 subunits, including APM-1 (μ1), UNC-101 (μ1), APB-1 (β1), APG-1 (γ), and APS-1 (σ1) (Shim et al., 2000; Zhou et al., 2016). Loss of APM-1 failed to cause uncoordinated (UNC) phenotypes (Shim et al., 2000; Zhou et al., 2016). Instead, UNC-101/AP-1 μ interacts with the bipartite signal within KVS-4/Kv2.1, mediating the polarized sorting of KVS-4 in DA9 neuron (Zhou et al., 2016). Regarding the functionality of clathrin, in addition to clathrin-coated pits during endocytosis, clathrin-coated vesicles also bud from TGN. Arf1 triggers the assembly of the clathrin coat on TGN (Thomas et al., 2021). A mechanistic study revealed that TGN-associated clathrin and AP-1 quickly exchange with free proteins in the cytoplasm, and AP-1 can exchange independently of clathrin (Wu et al., 2003). Together, these results suggested that AP-1 assembly and clathrin assembly are relatively independent events. Additional mechanisms are likely required to couple these two assembly processes, which remains to be elucidated.

Here, we introduced SMAP-1 (stromal membrane-associated protein-1) as a polarized secretion regulator in C. elegans intestinal epithelia. SMAP-1 overlapped well with TGN markers, AP-1, and clathrin. Notably, the presence of the AP-1 complex was essential for SMAP-1 localization. Furthermore, we found that SMAP-1 deficiency led to a loss of TGN localization of clathrin. Overexpression of clathrin instead of AP-1 component effectively alleviated secretion defects. In summary, our results suggested that AP-1 governs the TGN localization of SMAP-1, and SMAP-1 acts to facilitate clathrin assembly during polarized secretion.

Results

Loss of SMAP-1 Disturbs the Polarized Secretion in the Intestine

To better understand the regulatory mechanisms of polarized secretion in epithelia, we deployed apically localized ERM-1-GFP (a membrane-cytoskeleton linker) and basolateral SLCF-1-GFP (a monocarboxylate transporter) as cargos for a genome-wide RNAi screen. We found that loss of W09D10.1 led to defective secretion in the C. elegans intestine. W09D10.1 encodes the sole C. elegans homolog of mammalian SMAPs (Funaki et al., 2013; Sato et al., 1998; Tanabe et al., 2005), which we referred to as SMAP-1. Sequence alignment indicated that the N-terminal region (aa 15-119) of SMAP-1 shares 68% identity with SMAP2, while their C-terminal regions lack significant homology. SMAP-1 contains an N-terminal Arf GAP domain (aa 20-128) (Figure 1B). Unlike the C-terminus of SMAP2, which harbors a clathrin-interacting domain and a CALM-interacting domain (Natsume et al., 2006), the C-terminal part of SMAP-1 has not been characterized yet.

FIGURE 1
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FIGURE 1. Loss of SMAP-1 disturbs the polarized secretion in the intestine. (A) A model of the C. elegans intestine indicates the apical and basal sides of intestinal epithelia. (B) SMAP-1 contains an N-terminal ArfGAP domain, and amino acid numbers are indicated. (C-C′) In smap-1(ycxEx1639) mutants, ERM-1-GFP and NHX-2-GFP accumulated on the basolateral side, while SLCF-1-GFP and LET-413-GFP appeared on the apical membrane. White asterisks indicate intestinal lumen. Error bars are 95% CIs (n = 18 each). Asterisks designate the significant differences in the Mann-Whitney test (***p < 0.001). Scale bars, 10 μm.

In accordance with the predicted expression profile (Spencer et al., 2011), SMAP-1 is broadly expressed in tissues such as the intestine, neuron, and pharynx (Supplementary Figure S1). Whole-animal knockout of SMAP-1 causes larval arrest (Gonczy et al., 2000). Hence, we prepared the transgenic allele smap-1(ycxEx1639), a heat-shock-inducible CRISPR/Cas9 conditional mutant (Supplementary Figure S2A-A′). In smap-1(ycxEx1639) intestinal cells (Figures 1A,B), ERM-1-GFP consistently accumulated on the basal side, while SLCF-1 abnormally appeared on the apical membrane (Figure 1C-C′). Similarly, the localization of apical cargo protein NHX-2 (Na+/H+ exchanger) and basolateral recycling regulator LET-413/Erbin were affected (Figure 1C-C′). Previous studies suggested that SMAP2 could act as an Arf1GAP (Arf1 GTPase-activating protein) and regulate the formation of the clathrin coat on the trans-Golgi network (TGN) (Thomas et al., 2021), leading us to examine the distribution of GFP-ARF-1.2. In smap-1(ycxEx1639) cells, GFP-ARF-1.2 accumulated in punctate structures (Supplementary Figure S3A-A′), suggesting that SMAP-1 acts as a GAP of ARF-1.2 in intestinal cells. Consistently, overexpression of the SMAP-1 (R60A) variant that lost GAP activity was not sufficient to alleviate cytosolic overaccumulation of ARF-1.2-GFP in smap-1(ycxEx1639) cells (Supplementary Figure S3A-A′). The membrane-to-cytosol ratio of ARF-1.2-GFP was increased by ∼70% in smap-1(RNAi) animals (Supplementary Figure S3B-B′), and overexpressed SMAP-1-mCherry reduced the puncta labeling of ARF-1.2-GFP (Supplementary Figure S3A-A′).

To verify the role of ARF-1.2 in SMAP-mediated cargo sorting, we examined the localization of ERM-1 and SLCF-1. Notably, the distribution of ERM-1-GFP and SLCF-1-GFP was affected in arf-1.2(RNAi) animals (Supplementary Figure S3C-C′). However, ARF-1.2 knockdown failed to alleviate the distributional defects of ERM-1 and SLCF-1 in SMAP-1-deficient cells (Supplementary Figure S3C-C′). Conversely, overexpression of SMAP-1 (R60A)-mCherry rescued the distributional defects of ERM-1 and SLCF-1 (Supplementary Figure S3D-D′). It is noteworthy that Arf1 GTPase has been shown to recruit AP-1 to facilitate the formation of the clathrin coat on the trans-Golgi network (TGN) (Ren et al., 2013; Beacham et al., 2019). Altogether, our results suggested that in addition to acting as a GAP of ARF-1.2 during polarized cargo sorting, SMAP-1 plays an additional role in facilitating sorting in C. elegans intestinal epithelia.

ARF-1.2 is required for the retrograde transport from Golgi to the endoplasmic reticulum (ER) (Arakel et al., 2019). To this end, we set to examine the localization of COPB-1 (COP-I complex subunit beta 1), which is expressed in the intestine (Hunt-Newbury et al., 2007; Ackema et al., 2014). As expected, COPB-1 predominantly colocalized with Golgi marker mCherry-P4M (Supplementary Figure S4B-B′). In the absence of SMAP-1, the level of colocalization between COPB-1-GFP and MC-P4M was decreased significantly (Supplementary Figure S4B-B′). Similarly, the Golgi localization of COPG-1 (COP-I complex subunit gamma 1) was reduced (Supplementary Figure S4A-A′). These results indicated that the increase in ARF-1.2 activity induced by SMAP-1 deficiency affected the Golgi recruitment of COP-I. Alternatively, SMAP-1 could directly participate in the assembly of COP-I coatomer.

Additionally, Arf1 has been reported to promote the Golgi association of gamma ear-containing Arf-binding proteins (GGAs) (Doray et al., 2002; D'Souza-Schorey and Chavrier, 2006), which cooperates with clathrin in cargo sorting. In C. elegans, APT-9 is the homolog of human GGA1. To determine whether SMAP-1 affects the localization of APT-9/GGA1, we assessed the distribution of APT-9-GFP. In the absence of SMAP-1, although APT-9-GFP accumulated in the cytosol, the localization of APT-9-GFP in mCherry-P4M-labeled Golgi apparatus was significantly reduced (Supplementary Figure S4C-D′). These results suggested that SMAP-1 also functions as a negative regulator of APT-9/GGA1 in C. elegans intestinal cells. However, the increase in ARF-1.2 activity does not seem to affect the Golgi association of APT-9/GGA1 directly.

SMAP-1 Is Localized at the trans-Golgi Network

To characterize the intracellular position of SMAP-1, we compared mCherry-tagged SMAP-1 with a set of organelle markers. In the wild-type background, SMAP-1 localized to punctate structures in intestinal cells. In agreement with its functional implication, SMAP-1 overlapped with TGN marker GOLG-4/golgin-245 (Figures 2A,B) (Munro, 2011). AMAN-2 (alpha-mannosidase II) labels cis- and medial-Golgi (Sato et al., 2011). We observed an absence of colocalization between SMAP-1 and AMAN-2 (Figures 2A,B), which were often adjacent. Phosphoinositide PI(4)P is mainly enriched in the Golgi (Dickson et al., 2016). Consistently, SMAP-1 colocalized with PI(4)P marker GFP-P4M in cytosolic punctate structures (Supplementary Figure S5A,B). SMAP-1 was also juxtaposed to exocytosis-associated endosome markers RAB-11 and RAB-8 (Figures 2A,B) (Huber et al., 1993; Ang et al., 2003; Sato et al., 2008; Winter et al., 2012).

FIGURE 2
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FIGURE 2. SMAP-1 is localized at the trans-Golgi network. (A) SMAP-1 overlapped well with TGN marker GOLG-4/golgin-245. SMAP-1 and cis- and medial-Golgi marker AMAN-2 were often juxtaposed. SMAP-1 was also juxtaposed to endosome marker RAB-11 and RAB-8. Arrowheads designate structures co-labeled by GFP and mCherry. (B) Pearson’s correlation coefficients are calculated, error bars are 95% CIs (n = 12 animals). Scale bars, 10 μm.

Localization of SMAP-1 in the trans-Golgi Network Requires AP-1

Previous studies showed that the AP-1 complex mediates clathrin assembly and acts synergistically with clathrin to regulate sorting on the TGN (Robinson and Bonifacino, 2001). The punctate structures labeled by CHC-1 (clathrin heavy chain) were consistently reduced in the absence of AP-1 subunits (Figure 3A-A′). In addition, depleting AP-1 subunits or clathrin caused ERM-1 to accumulate around the basolateral membrane and resulted in the presence of SLCF-1 in the apical membrane of intestinal cells (Supplementary Figure S6A-A′). Remarkably, most SMAP-1-GFP-labeled structures also disappeared upon loss of AP-1 subunits (Figure 3A-A′). It is noteworthy that the C. elegans genome encodes an additional AP-1 μ1 subunit UNC-101 (Shim et al., 2000), which has been implicated in the polarized sorting of KVS-4 in DA9 motor neurons (Zhou et al., 2016). Conversely, loss of UNC-101 did not disturb the distributional of CHC-GFP (Supplementary Figure S6C-C′), supporting the distinct, tissue-specific functions of APM-1 and UNC-101 (Shim et al., 2000).

FIGURE 3
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FIGURE 3. The localization of SMAP-1 in the trans-Golgi network requires AP-1. (A-A′) In the middle focal plane, SMAP-1-GFP-labeled structures were reduced upon loss of AP-1 subunits. Also, the punctate structures labeled by CHC-1 (clathrin heavy chain) were decreased in the absence of AP-1 subunits. Error bars are 95% CIs (n = 18 each). Asterisks designate the significant differences in a one-way ANOVA followed by a post-hoc test (Dunn's Multiple Comparison Test) for multiple comparisons (***p < 0.001). (B-B′) Loss of SMAP-1 led to the accumulation of GFP-APG-1 and GFP-APB-1 on the punctate structures. In contrast, CHC-1-positive punctate structures were reduced in SMAP-1-deficient cells. Error bars are 95% CIs (n = 18 each). Asterisks designate the significant differences in the Mann-Whitney test (***p < 0.001). (C-C′) SMAP-1-mCherry colocalized well with CHC-1 and APB-1. Arrowheads designate structures co-labeled by GFP and mCherry. Pearson’s correlation coefficients are calculated, error bars are 95% CIs (n = 12 animals). (D–H) Western blot showing GST pull-down with in vitro translated HA-tagged proteins. GST-APG-1 exhibited interactions with HA-SMAP-1. Scale bars, 10 μm.

To further determine the genetic relationship between SMAP-1 and AP-1 or clathrin, we examined the distribution of clathrin and the AP-1 complex in smap-1 mutants. Of note, CHC-1-positive punctate structures decreased in SMAP-1-deficient cells (Figure 3B-B′). Large subunits APG-1 (γ subunit) and APB-1 (β1 subunit) are associated with the membrane and clathrin (Heldwein et al., 2004; Doray et al., 2007). Both GFP-APG-1 and GFP-APB-1 accumulated on the punctate structures in smap-1 mutants (Figure 3B-B′). Furthermore, we noticed that SMAP-1-mCherry colocalized with CHC-1, APB-1, and APM-1 (μ1 subunit) in intestinal cells (Figure 3C-C′; Supplementary Figure S6B-B′). To determine the interaction between SMAP-1 and AP-1 subunits APM-1 (μ1), APB-1 (β1), APG-1 (γ), and APS-1 (σ1), we performed GST pull-down assays. We found that SMAP-1 was bound to APG-1, while there was no significant interaction between SMAP-1 and APM-1, APB-1, and APS-1 (Figures 3D–G). In contrast, we did not observe the interaction between SMAP-1 and clathrin heavy chain (CHC-1) (Figure 3H). Together, these data suggested that the integrity of the AP-1 complex is required for SMAP-1 localization in TGN and that the clathrin assembly event likely occurs downstream of SMAP-1.

Next, we inspected the subcellular distribution of ERM-1-GFP and SLCF-1-GFP in SMAP-1 knockdown animals (Supplementary Figure S2B). As expected, overexpression of CHC-1 rescued the mislocalization phenotype of ERM-1 and SLCF-1 in the case of SMAP-1 deficiency (Figure 4A-B′). Conversely, the simultaneous overexpression of mCherry-tagged APM-1 (μ1), APB-1 (β1), APG-1 (γ), and APS-1 (σ1) failed to fully alleviate distribution defects of cargos in smap-1(RNAi) animals (Figure 4A-B′). Hence, our results suggested that SMAP-1 helps couple the AP-1 complex and clathrin in TGN-mediated sorting in the C. elegans intestine.

FIGURE 4
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FIGURE 4. Overexpression of CHC-1 relieved the mislocalization phenotype of ERM-1 and SLCF-1 in SMAP-1 knockdown animals. (A-A′) In smap-1(RNAi) mutants, overexpressed CHC-1 fully rescued the basolateral mislocalization phenotype of ERM-1. Error bars are 95% CIs (n = 18 each). Asterisks designate the significant differences in a one-way ANOVA followed by a post-hoc test (Dunn's Multiple Comparison Test) for multiple comparisons (***p < 0.001, **p < 0.01, *p < 0.05, ns: no significance). (B-B′) In smap-1(RNAi) mutants, overexpressed CHC-1 fully rescued the apical mislocalization phenotype of SLCF-1. Error bars are 95% CIs (n = 18 each). Asterisks designate the significant differences in a one-way ANOVA followed by a post-hoc test (Dunn's Multiple Comparison Test) for multiple comparisons (***p < 0.001, **p < 0.01, *p < 0.05, ns: no significance). Scale bars, 10 μm.

Loss of SMAP-1 Leads to Reduced Clathrin Coat Assembly in the TGN

Thus far, our analysis revealed that clathrin assembly is likely to occur downstream of SMAP-1. To further clarify the effect of SMAP-1 on clathrin localization, we compared clathrin with the PI(4)P marker GFP-P4M in the absence of SMAP-1. Remarkably, loss of SMAP-1 reduced the overlap between residual CHC-1-GFP and mCherry-P4M (Figure 5A-A′), validating that SMAP-1/SMAP2 regulates the occurrence of clathrin in the Golgi apparatus. However, the Golgi localization of APM-1 was not affected upon loss of SMAP-1 (Figure 5B-B′). We subsequently examined the level of colocalization between CHC-1-GFP and mCherry-APG-1. In the absence of SMAP-1, we found that the remaining CHC-1-labeled structure no longer colocalized with APG-1 (Figure 5C-C′). In addition to biosynthetic sorting, the clathrin coat is known to mediate the formation of endocytic clathrin-coated vesicles (Chen and Schmid, 2020; Moulay et al., 2020). Also, clathrin has been reported to function as a component of the retrograde transport machinery on the surface of the endosome (Saint-Pol et al., 2004; Shi et al., 2009). Therefore, the punctate structures distinct from the P4M- or APG-1-positive puncta are likely clathrin-coated vesicles and sorting endosomes (Figure 5A-A′, C-C′). Taken together, our results indicated that SMAP-1 acts as an indispensable regulator that directs TGN clathrin coat assembly downstream of the AP-1 complex.

FIGURE 5
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FIGURE 5. Loss of SMAP-1 leads to a decrease in TGN-located clathrin. (A-A′) Loss of SMAP-1 led to a decrease in the colocalization between CHC-1 and P4M. Pearson’s correlation coefficients are calculated, error bars are 95% CIs (n = 12 animals). p-value: Mann-Whitney test. ***p < 0.001. (B-B′) The colocalization between APM-1 and P4M was not affected by the depletion of SMAP-1. Pearson’s correlation coefficients are calculated, error bars are 95% CIs (n = 12 animals). p-value: Mann-Whitney test. ns: no significance. (C-C′) In the absence of SMAP-1, CHC-1 failed to overlap with APG-1 in punctate structures. Pearson’s correlation coefficients are calculated, error bars are 95% CIs (n = 12 animals). p-value: Mann-Whitney test. ***p < 0.001. Scale bars, 10 μm.

Discussion

Here, we identified SMAP-1/SMAPs as a polarized secretion regulator in the C. elegans intestine. SMAP-1 colocalizes with AP-1 and clathrin in the TGN. The integrity of the AP-1 complex is required for SMAP-1 positioning, and SMAP-1 acts to sustain clathrin assembly to ensure AP-1/clathrin-dependent cargos sorting (Supplementary Figure S7).

Studies in mammals indicated that SMAP1 functions as an Arf6GAP to regulate clathrin-dependent endocytosis via binding directly to clathrin (Tanabe et al., 2005). Additionally, SMAP2 was implicated in endosome-to-Golgi retrograde transport (Natsume et al., 2006). A recent study showed that SMAP2 facilitates clathrin assembly protein (CALM) mediated formation of clathrin-coated carriers on the TGN, promoting acrosome formation (Funaki et al., 2013). Together, these results suggested that SMAPs are clathrin assembly regulators in the TGN, and this efficacy could be due to its ArfGAP activity. In the current study, we found that SMAP-1 regulates polarized sorting, and this function seems independent of ARF-1.2, supporting the role of SMAPs as secretion regulators. Furthermore, our study highlighted the diversity of SMAPs functionality and corroborated the significance of AP-1/clathrin coat assembly in polarized sorting.

Previous studies have shown that SMAPs interact with clathrin and CALM, modulating clathrin-coated vesicle formation on the TGN (Tanabe et al., 2005; Natsume et al., 2006; Funaki et al., 2013). However, the mechanism controlling the localization of SMAPs is still not well understood. Here, by using a well-established in vivo membrane trafficking investigation model (Chen et al., 2018; Chen et al., 2019; Gao et al., 2020; Zhang et al., 2020; Yan et al., 2021), we showed that the integrity of AP-1 adaptor is necessary for the TGN positioning of SMAP-1. Although we did not specifically identify which AP-1 subunit governs the TGN localization of SMAP-1, our results suggested that in addition to CALM, SMAP-1 underlies an additional clathrin assembly mechanism, enriching the understanding of AP-1/clathrin coat assembly. It is reasonable to speculate that a similar mechanism might be involved in the budding of clathrin-coated vesicles during endocytosis. Further analyses are required to dissect the details of this biological process.

Materials and Methods

C. elegans Strains

Genetic crosses of C. elegans were performed by standard methods (Brenner, 1974). A list of strains was provided in Supplemental Materials. RNAi-mediated gene expression interference was implemented by the feeding protocol (Timmons and Fire, 1998). RNAi constructs were from the Ahringer library (Kamath and Ahringer, 2003). For chc-1, apb-1, apg-1, apm-1, and aps-1 RNAi experiments, L2-L3 stage larvae were cultured for 48–60h and scored as adults.

Antibodies

Mouse anti-α-Tubulin monoclonal antibody (T6199, Sigma, St. Louis, MO), and mouse anti-Flag monoclonal antibody (F1804; Sigma, St. Louis, MO) were used in this study.

CRISPR-Cas9 Mutant Strains

The CRISPR/Cas9 vectors were assembled by swapping the eft-3 promoter in pDD162 (Addgene, #47549) with the heat-shock promoter Phsp-16.2 (Shen et al., 2014; Li et al., 2015). CRISPR design tool (https://chopchop.cbu.uib.no/) was used to identify the knockout targets. Three smap-1 target sequences were selected, including AGG​TAA​AGT​GGA​TCC​GAA​GAa​gg, AAG​ACT​GCA​AGG​CTT​CCT​GTt​gg, AAA​AGT​GCG​CTC​AGT​GAA​TCt​gg. The CRISPR/Cas9 plasmids were validated by sequencing. CRISPR/Cas9 conditional knockout strains were created by microinjection of plasmids at 50 ng/μl and Podr-1:rfp (50 ng/μl) into wild-type hermaphrodites germline (Zhou et al., 2016). Heat-shock was executed at 0 h, 8 h, 16 h, 24 h, and 32 h after egg-hatching. The apical membrane cargo SLCF-1-GFP showed significant defects after heat shock at 0 h.

Plasmids and Transgenic Strains

For the SMAP-1 (R60A) rescue assay, a guide RNA (sgRNA) resistant plasmid was prepared by introducing silent mutations into each target sequence (5′-AGG​CAA​GGT​TGA​CCC​AAA​AAa​ag-3′, 5′-GAG​ACT​ACA​GGG​ATT​TCT​ATt​ag-3′, 5′-GAA​GGT​ACG​TTC​TGT​TAA​CCt​ag-3′). We also introduced a single missense mutation in the SMAP-1 GAP domain. To construct transgenes expressed explicitly in C. elegans intestine, the intestine-specific promoter vha-6 driven vectors modified with a Gateway cassette were deployed. The cDNA sequences of smap-1(w09d10.1), erm-1, slcf-1, nhx-2, chc-1, apb-1, apg-1, apm-1, aps-1, golg-4, P4M, and arf-1.2 lacking a stop codon or a start codon were cloned into intestinal vectors by LR reaction (Chen et al., 2006). Transgenic strains were generated by standard microinjection; plasmids were co-injected with selection markers Podr-1:gfp or Podr-1:rfp into wild-type or smap-1(ycxEx1639) hermaphrodites germ lines.

Worm Lysate Preparation and Western Blot

Around 100 wild-type or smap-1(RNAi) young adults (24 h after L4 stage) were picked into 20 μl lysis buffer [100 mM Tris pH 6.8, 8% SDS, 20 mM β-mercaptoethanol], then mixed with 20 μl 2xSDS-PAGE loading buffer and boiled at 100 °C for 10min. Lysates were resolved on SDS-PAGE [12% (wt/vol) polyacrylamide], blotted to nitrocellulose. After 5% milk blocking and washing, the membrane was blotted with anti-Flag and anti-Tubulin antibodies.

Microscopy and Image Analysis

Live animals were mounted on 2% agarose pads (100 mM levamisole). Fluorescence images were obtained with a Nikon C2 laser scanning confocal microscope (Nikon, Tokyo, Japan) equipped with a 100×N.A. 1.2 oil-immersion objective. Images were collected with NIS-Elements AR 4.40.00 software. Z-series of optical sections were acquired using 0.8–1 μm step size. Fluorescence data were evaluated with Metamorph software version 7.10.3.279 (Universal Imaging, West Chester, PA). The “Integrated Morphometry Analysis” component was utilized to assess the fluorescence intensity (total intensity), puncta number (structure count), and fluorescence area (total area) within unit regions. For each genotype, a total of 6 animals were analyzed in three unit regions of each intestine defined by a 100 × 100 (pixl2) box located randomly (n = 18 each). In this case, “total area” is a comprehensive parameter indicating the number and size of the fluorescent structures. Colocalization images were analyzed by Fiji (Image J) software (Schindelin et al., 2012). Pearson’s correlation coefficients were calculated with 6 animals for all genotypes.

Statistical Analysis

Prism software version 8.02 (GraphPad Software, La Jolla, CA) was deployed to perform statistical analyses.

Data Availability Statement

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.

Author Contributions

SW and AS designed the study; SW and LY performed the experiments; WZ, ZC, CH, HL, and YY contributed the reagents; SW and AS analyzed the data; SW and AS wrote the paper with inputs and final approval from all authors.

Funding

This work was supported by National Natural Science Foundation of China (32000489) to YY, National Science Fund for Distinguished Young Scholars (31825017), National Natural Science Foundation of China (32130027), National Key R&D Program of China (2017YFE0120300), and Major Research Plan of the Natural Science Foundation of China (91954001) to AS.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Acknowledgments

We are grateful to Xin Zhang, Xin Fu, and Zhenrong Yang for their assistance.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2021.774401/full#supplementary-material

Supplementary Figure S1 | Differential interference contrast (DIC) and fluorescence images showing that SMAP-1 is expressed in multiple tissues of C. elegans. (A) A GFP transgene driven by the smap-1 promoter in an adult hermaphrodite. (B) In the tail, arrows indicate neuron and intestine. (C) In the posterior region, arrows indicate intestine and ventral nerve cord (VNC). (D) In the head, arrowhead indicates pharynx. (E) In the mid-body, arrows indicate VNC and dorsal nerve cord (DNC). Scale bars, 10 μm.

Supplementary Figure S2 | (A-A′) Confocal images showing SLCF-1-GFP distribution in the intestinal cells. Heat-shock was executed at 0, 8, 16, 24, and 32 h after hatching. The SLCF-1-GFP localization was examined in young adult animals. Error bars are 95% CIs (n = 18 each, 9 animals of each genotype were sampled in whole-cell regions of two intestinal cells). Asterisk indicates the significant difference in a one-way ANOVA followed by a post-hoc test (Dunn's Multiple Comparison Test) for multiple comparisons (***p < 0.001, ns: no significance). (B) Western blot showing Flag-SMAP-1 levels in wild type and smap-1(RNAi) backgrounds. Scale bars, 10 μm.

Supplementary Figure S3 | (A-A′) Confocal images showing the subcellular localization of ARF-1.2-GFP. Black asterisks in the panels indicate intestinal lumen. Error bars are 95% CIs (n = 18 each, 9 animals of each genotype were sampled in whole-cell regions of two intestinal cells). Asterisks indicate the significant differences in the Mann-Whitney test (***p < 0.001, *p < 0.05). (B-B′) The membrane-to-cytosol ratio (P/S) of ARF-1.2-GFP increased in smap-1(RNAi) animals. Membrane structures were separated from the cytosol of worm lysates by ultracentrifugation. ARF-1.2-GFP in the supernatants and pellets were analyzed by western blotting using an anti-GFP antibody. The loading control was blotted by the anti-Tubulin antibody. The SEMs from three independent experiments are shown, asterisk indicates the significant differences in the one-tailed Student’s t-test (***p < 0.001). (C-C′) Confocal images of the intestinal cells expressing GFP-tagged ERM-1 and SLCF-1. Asterisks in the panels indicate intestinal lumen. Error bars are 95% CIs (n = 18 each, 9 animals of each genotype were sampled in whole-cell regions of two intestinal cells). Asterisks indicate the significant differences in the Mann-Whitney test (***p < 0.001, ns: no significance). (D-D′) Confocal images of the intestinal cells expressing GFP-tagged ERM-1 and SLCF-1. In smap-1(ycxEx1639) mutants, overexpression of SMAP-1(R60A)-mCherry that has lost GAP activity rescued the distribution defects of ERM-1-GFP and SLCF-1-GFP. Asterisks in the panels indicate intestinal lumen. Error bars are 95% CIs (n = 18 each, 9 animals of each genotype were sampled in whole-cell regions of two intestinal cells). Asterisks indicate the significant differences in the Mann-Whitney test (***p < 0.001, ns: no significance). Scale bars, 10 μm.

Supplementary Figure S4 | (A-A′) Confocal images showing colocalization between COPG-1 and Golgi marker P4M in the intestinal cells. Arrowheads indicate structures labeled by both GFP and mCherry. Pearson’s correlation coefficients for GFP and mCherry signals are calculated, error bars are 95% CIs (n = 12 animals). P-value: Mann-Whitney test. ***p < 0.001. (B-B′) Confocal images showing colocalization between COPB-1 and Golgi marker P4M in the intestinal cells. Arrowheads indicate structures labeled by both GFP and mCherry. Pearson’s correlation coefficients for GFP and mCherry signals are calculated, error bars are 95% CIs (n = 12 animals). P-value: Mann-Whitney test. ***p < 0.001. (C-C′) Confocal images showing colocalization between APT-9 and Golgi marker P4M in the intestinal cells. Arrowheads indicate structures labeled by both GFP and mCherry. Pearson’s correlation coefficients for GFP and mCherry signals are calculated, error bars are 95% CIs (n = 12 animals). P-value: Mann-Whitney test. ***p < 0.001. (D-D′) Confocal images showing APT-9-GFP in the intestinal cells. Error bars are 95% CIs (n = 18 each, 9 animals of each genotype were sampled in whole-cell regions of two intestinal cells). Asterisks indicate the significant differences in the Mann-Whitney test (***p < 0.001). Scale bars, 10 μm.

Supplementary Figure S5 | (A) Confocal image showing colocalization between PI(4)P marker GFP-P4M and SMAP-1-mCherry in the intestinal cells. Arrowheads indicate structures labeled by both GFP and mCherry. (B) Pearson’s correlation coefficients for GFP and mCherry signals are calculated, error bars are 95% CIs (n = 12 animals). Scale bar represents 10 μm.

Supplementary Figure S6 | (A-A′) Confocal images of the intestinal cells expressing GFP-tagged ERM-1 and SLCF-1. Asterisks in the panels indicate intestinal lumen. Error bars are 95% CIs (n = 18 each, 9 animals of each genotype were sampled in whole-cell regions of two intestinal cells). Asterisks indicate the significant differences in a one-way ANOVA followed by a post-hoc test (Dunn's Multiple Comparison Test) for multiple comparisons (***p < 0.001). (B-B′) Confocal image showing colocalization between GFP-APM-1 (μ1 subunit) and SMAP-1-mCherry in the intestinal cells. Arrowheads indicate structures labeled by both GFP and mCherry. Pearson’s correlation coefficients for GFP and mCherry signals are calculated, error bars are 95% CIs (n = 12 animals). (C-C′) Confocal images showing CHC-1-GFP in the intestinal cells. Error bars are 95% CIs (n = 18 each, 9 animals of each genotype were sampled in whole-cell regions of two intestinal cells). Asterisks indicate the significant differences in the Mann-Whitney test (ns: no significance). Scale bars, 10 μm.

Supplementary Figure S7 | In the C. elegans intestine, ARF-1.2 regulates clathrin coat assembly via downstream AP-1 and SMAP-1, promoting TGN sorting. In addition, SMAP-1 acts as a GAP to regulate ARF-1.2 activity in a negative feedback manner.

References

Ackema, K. B., Hench, J., Böckler, S., Wang, S. C., Sauder, U., Mergentaler, H., et al. (2014). The Small GTP Ase Arf1 Modulates Mitochondrial Morphology and Function. EMBO J. 33, 2659–2675. doi:10.15252/embj.201489039

PubMed Abstract | CrossRef Full Text | Google Scholar

Ang, A. L., Folsch, H., Koivisto, U.-M., Pypaert, M., and Mellman, I. (2003). The Rab8 GTPase Selectively Regulates AP-1b-dependent Basolateral Transport in Polarized Madin-Darby Canine Kidney Cells. J. Cel Biol. 163, 339–350. doi:10.1083/jcb.200307046

PubMed Abstract | CrossRef Full Text | Google Scholar

Arakel, E. C., Huranova, M., Estrada, A. F., Rau, E. M., Spang, A., and Schwappach, B. (2019). Dissection of GTPase Activating Proteins Reveals Functional Asymmetry in the COPI Coat. J. Cel Sci. 132 (16), jcs232124. doi:10.1242/jcs.232124

CrossRef Full Text | Google Scholar

Beacham, G. M., Partlow, E. A., and Hollopeter, G. (2019). Conformational Regulation of AP1 and AP2 Clathrin Adaptor Complexes. Traffic 20, 741–751. doi:10.1111/tra.12677

PubMed Abstract | CrossRef Full Text | Google Scholar

Brenner, S. (1974). The Genetics of Caenorhabditis elegans. Genetics 77, 71–94. doi:10.1093/genetics/77.1.71

PubMed Abstract | CrossRef Full Text | Google Scholar

Brodsky, F. M., Chen, C.-Y., Knuehl, C., Towler, M. C., and Wakeham, D. E. (2001). Biological Basket Weaving: Formation and Function of Clathrin-Coated Vesicles. Annu. Rev. Cel Dev. Biol. 17, 517–568. doi:10.1146/annurev.cellbio.17.1.517

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, C. C.-H., Schweinsberg, P. J., Vashist, S., Mareiniss, D. P., Lambie, E. J., and Grant, B. D. (2006). RAB-10 Is Required for Endocytic Recycling in theCaenorhabditis elegansIntestine. MBoC 17, 1286–1297. doi:10.1091/mbc.e05-08-0787

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, D., Xu, W., Wang, Y., Ye, Y., Wang, Y., Yu, M., et al. (2019). Revealing Functional Crosstalk between Distinct Bioprocesses through Reciprocal Functional Tests of Genetically Interacting Genes. Cel Rep. 29, 2646–2658. doi:10.1016/j.celrep.2019.10.076

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, D., Yang, C., Liu, S., Hang, W., Wang, X., Chen, J., et al. (2018). SAC-1 Ensures Epithelial Endocytic Recycling by Restricting ARF-6 Activity. J. Cel Biol. 217, 2121–2139. doi:10.1083/jcb.201711065

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, Z., and Schmid, S. L. (2020). Evolving Models for Assembling and Shaping Clathrin-Coated Pits. J. Cel Biol. 219 (9), e202005126. doi:10.1083/jcb.202005126

CrossRef Full Text | Google Scholar

D'Souza-Schorey, C., and Chavrier, P. (2006). ARF Proteins: Roles in Membrane Traffic and beyond. Nat. Rev. Mol. Cel Biol. 7, 347–358. doi:10.1038/nrm1910

CrossRef Full Text | Google Scholar

Dickson, E. J., Jensen, J. B., Vivas, O., Kruse, M., Traynor-Kaplan, A. E., and Hille, B. (2016). Dynamic Formation of ER-PM Junctions Presents a Lipid Phosphatase to Regulate Phosphoinositides. J. Cel Biol. 213, 33–48. doi:10.1083/jcb.201508106

CrossRef Full Text | Google Scholar

Dimou, E., and Nickel, W. (2018). Unconventional Mechanisms of Eukaryotic Protein Secretion. Curr. Biol. 28, R406–r410. doi:10.1016/j.cub.2017.11.074

PubMed Abstract | CrossRef Full Text | Google Scholar

Doray, B., Ghosh, P., Griffith, J., Geuze, H. J., and Kornfeld, S. (2002). Cooperation of GGAs and AP-1 in Packaging MPRs at the Trans-golgi Network. Science 297, 1700–1703. doi:10.1126/science.1075327

PubMed Abstract | CrossRef Full Text | Google Scholar

Doray, B., Lee, I., Knisely, J., Bu, G., and Kornfeld, S. (2007). The γ/σ1 and α/σ2 Hemicomplexes of Clathrin Adaptors AP-1 and AP-2 Harbor the Dileucine Recognition Site. MBoC 18, 1887–1896. doi:10.1091/mbc.e07-01-0012

PubMed Abstract | CrossRef Full Text | Google Scholar

Fölsch, H., Pypaert, M., Maday, S., Pelletier, L., and Mellman, I. (2003). The AP-1A and AP-1B Clathrin Adaptor Complexes Define Biochemically and Functionally Distinct Membrane Domains. J. Cel Biol. 163, 351–362. doi:10.1083/jcb.200309020

CrossRef Full Text | Google Scholar

Funaki, T., Kon, S., Tanabe, K., Natsume, W., Sato, S., Shimizu, T., et al. (2013). The Arf GAP SMAP2 Is Necessary for Organized Vesicle Budding from the Trans-golgi Network and Subsequent Acrosome Formation in Spermiogenesis. MBoC 24, 2633–2644. doi:10.1091/mbc.e13-05-0234

PubMed Abstract | CrossRef Full Text | Google Scholar

Gao, J., Zhao, L., Luo, Q., Liu, S., Lin, Z., Wang, P., et al. (2020). An EHBP-1-SID-3-DYN-1 axis Promotes Membranous Tubule Fission during Endocytic Recycling. Plos Genet. 16, e1008763. doi:10.1371/journal.pgen.1008763

PubMed Abstract | CrossRef Full Text | Google Scholar

Gönczy, P., Echeverri, C., Oegema, K., Coulson, A., Jones, S. J. M., Copley, R. R., et al. (2000). Functional Genomic Analysis of Cell Division in C. elegans Using RNAi of Genes on Chromosome III. Nature 408, 331–336. doi:10.1038/35042526

PubMed Abstract | CrossRef Full Text | Google Scholar

Gravotta, D., Deora, A., Perret, E., Oyanadel, C., Soza, A., Schreiner, R., et al. (2007). AP1B Sorts Basolateral Proteins in Recycling and Biosynthetic Routes of MDCK Cells. Proc. Natl. Acad. Sci. 104, 1564–1569. doi:10.1073/pnas.0610700104

PubMed Abstract | CrossRef Full Text | Google Scholar

Guo, Y., Sirkis, D. W., and Schekman, R. (2014). Protein Sorting at the Trans-golgi Network. Annu. Rev. Cel Dev. Biol. 30, 169–206. doi:10.1146/annurev-cellbio-100913-013012

PubMed Abstract | CrossRef Full Text | Google Scholar

Heldwein, E. E., Macia, E., Wang, J., Yin, H. L., Kirchhausen, T., and Harrison, S. C. (2004). Crystal Structure of the Clathrin Adaptor Protein 1 Core. Proc. Natl. Acad. Sci. 101, 14108–14113. doi:10.1073/pnas.0406102101

PubMed Abstract | CrossRef Full Text | Google Scholar

Huber, L. A., Pimplikar, S., Parton, R. G., Virta, H., Zerial, M., and Simons, K. (1993). Rab8, a Small GTPase Involved in Vesicular Traffic between the TGN and the Basolateral Plasma Membrane. J. Cel Biol. 123, 35–45. doi:10.1083/jcb.123.1.35

PubMed Abstract | CrossRef Full Text | Google Scholar

Hunt-Newbury, R., Viveiros, R., Johnsen, R., Mah, A., Anastas, D., Fang, L., et al. (2007). High-throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans. Plos Biol. 5, e237. doi:10.1371/journal.pbio.0050237

PubMed Abstract | CrossRef Full Text | Google Scholar

Kamath, R., and Ahringer, J. (2003). Genome-wide RNAi Screening in Caenorhabditis elegans. Methods 30, 313–321. doi:10.1016/s1046-2023(03)00050-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, W., Yi, P., and Ou, G. (2015). Somatic CRISPR-Cas9-Induced Mutations Reveal Roles of Embryonically Essential Dynein Chains in Caenorhabditis elegans Cilia. J. Cel Biol. 208, 683–692. doi:10.1083/jcb.201411041

CrossRef Full Text | Google Scholar

Mellman, I., and Warren, G. (2000). The Road Taken. Cell 100, 99–112. doi:10.1016/s0092-8674(00)81687-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Moulay, G., Lainé, J., Lemaître, M., Nakamori, M., Nishino, I., Caillol, G., et al. (2020). Alternative Splicing of Clathrin Heavy Chain Contributes to the Switch from Coated Pits to Plaques. J. Cel Biol. 219 (9), e201912061. doi:10.1083/jcb.201912061

CrossRef Full Text | Google Scholar

Munro, S. (2011). The Golgin Coiled-Coil Proteins of the Golgi Apparatus. Cold Spring Harb Perspect. Biol. 3 (6), a005256. doi:10.1101/cshperspect.a005256

PubMed Abstract | CrossRef Full Text | Google Scholar

Nakajo, A., Yoshimura, S.-i., Togawa, H., Kunii, M., Iwano, T., Izumi, A., et al. (2016). EHBP1L1 Coordinates Rab8 and Bin1 to Regulate Apical-Directed Transport in Polarized Epithelial Cells. J. Cel Biol. 212, 297–306. doi:10.1083/jcb.201508086

PubMed Abstract | CrossRef Full Text | Google Scholar

Nakatsu, F., Hase, K., and Ohno, H. (2014). The Role of the Clathrin Adaptor AP-1: Polarized Sorting and beyond. Membranes 4, 747–763. doi:10.3390/membranes4040747

PubMed Abstract | CrossRef Full Text | Google Scholar

Natsume, W., Tanabe, K., Kon, S., Yoshida, N., Watanabe, T., Torii, T., et al. (2006). SMAP2, a Novel ARF GTPase-Activating Protein, Interacts with Clathrin and Clathrin Assembly Protein and Functions on the AP-1-Positive Early Endosome/trans-Golgi Network. MBoC 17, 2592–2603. doi:10.1091/mbc.e05-10-0909

PubMed Abstract | CrossRef Full Text | Google Scholar

Rabouille, C. (2017). Pathways of Unconventional Protein Secretion. Trends Cel Biol. 27, 230–240. doi:10.1016/j.tcb.2016.11.007

PubMed Abstract | CrossRef Full Text | Google Scholar

Ren, X., Farías, G. G., Canagarajah, B. J., Bonifacino, J. S., and Hurley, J. H. (2013). Structural Basis for Recruitment and Activation of the AP-1 Clathrin Adaptor Complex by Arf1. Cell 152, 755–767. doi:10.1016/j.cell.2012.12.042

PubMed Abstract | CrossRef Full Text | Google Scholar

Robinson, M. S., and Bonifacino, J. S. (2001). Adaptor-related Proteins. Curr. Opin. Cel Biol. 13, 444–453. doi:10.1016/s0955-0674(00)00235-0

CrossRef Full Text | Google Scholar

Saint-Pol, A., Yélamos, B., Amessou, M., Mills, I. G., Dugast, M., Tenza, D., et al. (2004). Clathrin Adaptor epsinR Is Required for Retrograde Sorting on Early Endosomal Membranes. Dev. Cel. 6, 525–538. doi:10.1016/s1534-5807(04)00100-5

CrossRef Full Text | Google Scholar

Sato, M., Grant, B. D., Harada, A., and Sato, K. (2008). Rab11 Is Required for Synchronous Secretion of Chondroitin Proteoglycans after Fertilization inCaenorhabditis Elegans. J. Cel Sci. 121, 3177–3186. doi:10.1242/jcs.034678

CrossRef Full Text | Google Scholar

Sato, M., Saegusa, K., Sato, K., Hara, T., Harada, A., and Sato, K. (2011). Caenorhabditis elegans SNAP-29 Is Required for Organellar Integrity of the Endomembrane System and General Exocytosis in Intestinal Epithelial Cells. MBoC 22, 2579–2587. doi:10.1091/mbc.e11-04-0279

PubMed Abstract | CrossRef Full Text | Google Scholar

Sato, T., Mushiake, S., Kato, Y., Sato, K., Sato, M., Takeda, N., et al. (2007). The Rab8 GTPase Regulates Apical Protein Localization in Intestinal Cells. Nature 448, 366–369. doi:10.1038/nature05929

PubMed Abstract | CrossRef Full Text | Google Scholar

Sato, Y., Hong, H. N., Yanai, N., and Obinata, M. (1998). Involvement of Stromal Membrane-Associated Protein (SMAP-1) in Erythropoietic Microenvironment. J. Biochem. 124, 209–216. doi:10.1093/oxfordjournals.jbchem.a022082

PubMed Abstract | CrossRef Full Text | Google Scholar

Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., et al. (2012). Fiji: an Open-Source Platform for Biological-Image Analysis. Nat. Methods 9, 676–682. doi:10.1038/nmeth.2019

PubMed Abstract | CrossRef Full Text | Google Scholar

Shen, Z., Zhang, X., Chai, Y., Zhu, Z., Yi, P., Feng, G., et al. (2014). Conditional Knockouts Generated by Engineered CRISPR-Cas9 Endonuclease Reveal the Roles of Coronin in C. elegans Neural Development. Dev. Cel. 30, 625–636. doi:10.1016/j.devcel.2014.07.017

PubMed Abstract | CrossRef Full Text | Google Scholar

Shi, A., Sun, L., Banerjee, R., Tobin, M., Zhang, Y., and Grant, B. D. (2009). Regulation of Endosomal Clathrin and Retromer-Mediated Endosome to Golgi Retrograde Transport by the J-Domain Protein RME-8. EMBO J. 28, 3290–3302. doi:10.1038/emboj.2009.272

PubMed Abstract | CrossRef Full Text | Google Scholar

Shim, J., Sternberg, P. W., and Lee, J. (2000). Distinct and Redundant Functions of μ1 Medium Chains of the AP-1 Clathrin-Associated Protein Complex in the NematodeCaenorhabditis Elegans. MBoC 11, 2743–2756. doi:10.1091/mbc.11.8.2743

PubMed Abstract | CrossRef Full Text | Google Scholar

Shteyn, E., Pigati, L., and Fölsch, H. (2011). Arf6 Regulates AP-1b-dependent Sorting in Polarized Epithelial Cells. J. Cel Biol. 194, 873–887. doi:10.1083/jcb.201106010

CrossRef Full Text | Google Scholar

Spencer, W. C., Zeller, G., Watson, J. D., Henz, S. R., Watkins, K. L., Mcwhirter, R. D., et al. (2011). A Spatial and Temporal Map of C. elegans Gene Expression. Genome Res. 21, 325–341. doi:10.1101/gr.114595.110

PubMed Abstract | CrossRef Full Text | Google Scholar

Tanabe, K., Torii, T., Natsume, W., Braesch-Andersen, S., Watanabe, T., and Satake, M. (2005). A Novel GTPase-Activating Protein for ARF6 Directly Interacts with Clathrin and Regulates Clathrin-dependent Endocytosis. MBoC 16, 1617–1628. doi:10.1091/mbc.e04-08-0683

PubMed Abstract | CrossRef Full Text | Google Scholar

Thomas, L. L., Highland, C. M., and Fromme, J. C. (2021). Arf1 Orchestrates Rab GTPase Conversion at the Trans-golgi Network. Mol. Biol. Cel 32 (11), 1104–1120. doi:10.1091/mbc.e20-10-0664

CrossRef Full Text | Google Scholar

Thuenauer, R., Hsu, Y.-C., Carvajal-Gonzalez, J. M., Deborde, S., Chuang, J.-Z., Romer, W., et al. (2014). Four-dimensional Live Imaging of Apical Biosynthetic Trafficking Reveals a post-Golgi Sorting Role of Apical Endosomal Intermediates. Proc. Natl. Acad. Sci. 111, 4127–4132. doi:10.1073/pnas.1304168111

PubMed Abstract | CrossRef Full Text | Google Scholar

Timmons, L., and Fire, A. (1998). Specific Interference by Ingested dsRNA. Nature 395, 854. doi:10.1038/27579

PubMed Abstract | CrossRef Full Text | Google Scholar

Winter, J. F., Höpfner, S., Korn, K., Farnung, B. O., Bradshaw, C. R., Marsico, G., et al. (2012). Caenorhabditis elegans Screen Reveals Role of PAR-5 in RAB-11-Recycling Endosome Positioning and Apicobasal Cell Polarity. Nat. Cel Biol. 14, 666–676. doi:10.1038/ncb2508

PubMed Abstract | CrossRef Full Text | Google Scholar

Wu, X., Zhao, X., Puertollano, R., Bonifacino, J. S., Eisenberg, E., and Greene, L. E. (2003). Adaptor and Clathrin Exchange at the Plasma Membrane Andtrans-Golgi Network. MBoC 14, 516–528. doi:10.1091/mbc.e02-06-0353

PubMed Abstract | CrossRef Full Text | Google Scholar

Yan, Y., Liu, S., Hu, C., Xie, C., Zhao, L., Wang, S., et al. (2021). RTKN-1/Rhotekin Shields Endosome-Associated F-Actin from Disassembly to Ensure Endocytic Recycling. J. Cel Biol 220, e202007149. doi:10.1083/jcb.202007149

CrossRef Full Text | Google Scholar

Yeaman, C., Grindstaff, K. K., and Nelson, W. J. (1999). New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity. Physiol. Rev. 79, 73–98. doi:10.1152/physrev.1999.79.1.73

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, W., Wang, S., Yang, C., Hu, C., Chen, D., Luo, Q., et al. (2020). LET-502/ROCK Regulates Endocytic Recycling by Promoting Activation of RAB-5 in a Distinct Subpopulation of Sorting Endosomes. Cel Rep. 32, 108173. doi:10.1016/j.celrep.2020.108173

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhou, X., Zeng, J., Ouyang, C., Luo, Q., Yu, M., Yang, Z., et al. (2016). A Novel Bipartite UNC-101/AP-1 μ1 Binding Signal Mediates KVS-4/Kv2.1 Somatodendritic Distribution inCaenorhabditis Elegans. FEBS Lett. 590, 76–92. doi:10.1002/1873-3468.12043

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: C. elegans, polarized sorting, SMAP-1/SMAPs, AP-1, clathrin, intestinal epithelia

Citation: Wang S, Yao L, Zhang W, Cheng Z, Hu C, Liu H, Yan Y and Shi A (2021) AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia. Front. Cell Dev. Biol. 9:774401. doi: 10.3389/fcell.2021.774401

Received: 11 September 2021; Accepted: 05 November 2021;
Published: 25 November 2021.

Edited by:

Huijie Bian, Fourth Military Medical University, China

Reviewed by:

Satoshi Kametaka, Nagoya University, Japan
Matthew Seaman, University of Cambridge, United Kingdom

Copyright © 2021 Wang, Yao, Zhang, Cheng, Hu, Liu, Yan and Shi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Anbing Shi, ashi@hust.edu.cn

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