AUTHOR=Jiang Mengmeng , Xiao Yanyu , E Weigao , Ma Lifeng , Wang Jingjing , Chen Haide , Gao Ce , Liao Yuan , Guo Qile , Peng Jinrong , Han Xiaoping , Guo Guoji TITLE=Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=9 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.743421 DOI=10.3389/fcell.2021.743421 ISSN=2296-634X ABSTRACT=

Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single cells covering major zebrafish cell types and constructed a systematic zebrafish cell landscape. We revealed single-cell compositions for 18 zebrafish tissue types covering both embryo and adult stages. Single-cell mapping of caudal fin regeneration revealed a unique characteristic of blastema population and key genetic regulation involved in zebrafish tissue repair. Overall, our single-cell datasets demonstrate the utility of zebrafish cell landscape resources in various fields of biological research.