AUTHOR=Liu Yi , Liu Xiaolei , Yang Li , Qiu Yangyuan , Pang Jianda , Hu Xiaoxiang , Dong Zijian , Liu Zengshan , Jin Xuemin 

TITLE=Adjuvanticity of β -Glucan for Vaccine Against Trichinella spiralis

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.701708

DOI=10.3389/fcell.2021.701708

ISSN=2296-634X

ABSTRACT=<p>In the past 30 years, few researches focus on the efficacy of adjuvant against <italic>Trichinella spiralis</italic> infection. Identifying new, improved vaccine adjuvants for <italic>T. spiralis</italic> infection are required. β-glucan are effective and safe as adjuvant for infectious diseases. In this paper, we first observed the adjuvanticity of β-glucan as adjuvant for defensing helminth <italic>T. spiralis in vivo</italic>. We showed that IgG and IgE were elevated in the mice immunized with β-glucan combined with recombinant <italic>T. spiralis</italic> serine protease inhibitor (r<italic>Ts</italic>-Serpin), which is one of the vaccine candidates. Furthermore, <italic>in vitro</italic>, the combination of β-glucan and r<italic>Ts</italic>-Serpin enhanced the maturation of bone marrow dendritic cells (BMDCs) compared to r<italic>Ts</italic>-Serpin alone. We showed that β-glucan + r<italic>Ts</italic>-Serpin –treated BMDCs secreted higher production of IL-12 and IL-10. Moreover, β-glucan + r<italic>Ts</italic>-Serpin –treated BMDCs not only promoted the population of CD4<sup>+</sup> IFN-γ<sup>+</sup> T cells, but also enhanced the population of CD4<sup>+</sup> IL-4<sup>+</sup> T cells. These findings suggested that β-glucan, as an adjuvant, have the capacity to protect against <italic>T. spiralis</italic> infection via activating both Th1 and Th2 immune response.</p>