AUTHOR=Li Zhaoqing , Zhuo Wenying , Chen Lini , Zhang Xun , Chen Cong , Hu Dengdi , Chen Yongxia , Yang Jingjing , Zhou Yulu , Mao Misha , Xu Ling , Ju Siwei , Shen Jun , Wang Qinchuan , Dong Minjun , Xie Shuduo , Zhou Jichun , Wang Linbo TITLE=Establishment and Characterization of a HER2-Positive Cell Line Derived From the Pleural Effusion of a Drug-Resistant Breast Cancer Patient JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=9 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.680968 DOI=10.3389/fcell.2021.680968 ISSN=2296-634X ABSTRACT=

Drug resistance is a daunting challenge in the treatment of breast cancer, making it an urgent problem to solve in studies. Cell lines are important tools in basic and preclinical studies; however, few breast cell lines from drug-resistant patients are available. Herein, we established a novel HER2-positive breast cancer cell line from the pleural effusion of a drug-resistant metastatic breast cancer patient. This cell line has potent proliferative capability and tumorigenicity in nude mice but weak invasive and colony-forming capability. The molecular subtype of the cell line and its sensitivity to chemotherapeutics and HER2-targeting agents are different from those of its origin, suggesting that the phenotype changes between the primary and metastatic forms of breast cancer.