AUTHOR=Wang Chunli , Liu Songcai , Li Jiaxin , Cheng Yunyun , Wang Zhaoguo , Feng Tianqi , Lu Guanhong , Wang Siyao , Song Jie , Xia Peijun , Hao Linlin TITLE=Biological Functions of Let-7e-5p in Promoting the Differentiation of MC3T3-E1 Cells JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=9 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.671170 DOI=10.3389/fcell.2021.671170 ISSN=2296-634X ABSTRACT=
MicroRNAs let-7c and let-7f, two members of the let-7 family, were involved in regulating osteoblast differentiation and have an important role in bone formation. Let-7e-5p, which also belonged to the let-7 family, presented in the differentiation of adipose-derived stem cells and mouse embryonic stem cells. However, the role of let-7e-5p in osteoblast differentiation was unclear. Thus, this study aimed to elucidate the function of let-7e-5p in osteoblast differentiation and its mechanism. Firstly, we found that the let-7e-5p mimic promoted osteoblast differentiation but not the proliferation of MC3T3-E1 cells by positively regulating the expression levels of osteogenic-associated genes (