AUTHOR=Shin Thomas H. , Theodorou Evangelos , Holland Carl , Yamin Rae’e , Raggio Cathleen L. , Giampietro Philip F. , Sweetser David A. 

TITLE=TLE4 Is a Critical Mediator of Osteoblast and Runx2-Dependent Bone Development

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.671029

DOI=10.3389/fcell.2021.671029

ISSN=2296-634X

ABSTRACT=<p>Healthy bone homeostasis hinges upon a delicate balance and regulation of multiple processes that contribute to bone development and metabolism. While examining hematopoietic regulation by <italic>Tle4</italic>, we have uncovered a previously unappreciated role of <italic>Tle4</italic> on bone calcification using a novel <italic>Tle4</italic> null mouse model. Given the significance of osteoblasts in both hematopoiesis and bone development, this study investigated how loss of <italic>Tle4</italic> affects osteoblast function. We used dynamic bone formation parameters and microCT to characterize the adverse effects of <italic>Tle4</italic> loss on bone development. We further demonstrated loss of <italic>Tle4</italic> impacts expression of several key osteoblastogenic genes, including <italic>Runx2</italic>, <italic>Oc</italic>, and <italic>Ap</italic>, pointing toward a potential novel mechanism for <italic>Tle4</italic>-dependent regulation of mammalian bone development in collaboration with the RUNX family members.</p>