AUTHOR=Zhang Pan , Qian Dong , Luo Changxin , Niu Yingzhi , Li Tian , Li Chengying , Xiang Yun , Wang Xinyu , Niu Yue 

TITLE=Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.635533

DOI=10.3389/fcell.2021.635533

ISSN=2296-634X

ABSTRACT=<p>Low temperature is a major adverse environment that affects normal plant growth. Previous reports showed that the actin cytoskeleton plays an important role in the plant response to low-temperature stress, but the regulatory mechanism of the actin cytoskeleton in this process is not clear. C-repeat binding factors (CBFs) are the key molecular switches for plants to adapt to cold stress. However, whether CBFs are involved in the regulation of the actin cytoskeleton has not been reported. We found that <italic>Arabidopsis actin depolymerizing factor 5</italic> (<italic>ADF5</italic>), an ADF that evolved F-actin bundling function, was up-regulated at low temperatures. We also demonstrated that CBFs bound to the <italic>ADF5</italic> promoter directly <italic>in vivo</italic> and <italic>in vitro</italic>. The cold-induced expression of <italic>ADF5</italic> was significantly inhibited in the <italic>cbfs</italic> triple mutant. The freezing resistance of the <italic>adf5</italic> knockout mutant was weaker than that of wild type (WT) with or without cold acclimation. After low-temperature treatment, the actin cytoskeleton of WT was relatively stable, but the actin cytoskeletons of <italic>adf5, cbfs</italic>, and <italic>adf5 cbfs</italic> were disturbed to varying degrees. Compared to WT, the endocytosis rate of the amphiphilic styryl dye FM4-64 in <italic>adf5, cbfs</italic>, and <italic>adf5 cbfs</italic> at low temperature was significantly reduced. In conclusion, CBFs directly combine with the CRT/DRE DNA regulatory element of the <italic>ADF5</italic> promoter after low-temperature stress to transcriptionally activate the expression of <italic>ADF5</italic>; ADF5 further regulates the actin cytoskeleton dynamics to participate in the regulation of plant adaptation to a low-temperature environment.</p>