AUTHOR=Royo Felix , Azkargorta Mikel , Lavin Jose L. , Clos-Garcia Marc , Cortazar Ana R. , Gonzalez-Lopez Monika , Barcena Laura , del Portillo Hernando A. , Yáñez-Mó María , Marcilla Antonio , Borras Francesc E. , Peinado Hector , Guerrero Isabel , Váles-Gómez Mar , Cereijo Unai , Sardon Teresa , Aransay Ana M. , Elortza Felix , Falcon-Perez Juan M. 

TITLE=Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.613583

DOI=10.3389/fcell.2020.613583

ISSN=2296-634X

ABSTRACT=<p>Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.</p>