AUTHOR=Guo Tianyu , Wang Yang , Jia Jing , Mao Xueying , Stankiewicz Elzbieta , Scandura Glenda , Burke Edwina , Xu Lei , Marzec Jacek , Davies Caitlin R. , Lu Jiaying Jasmin , Rajan Prabhakar , Grey Alistair , Tipples Karen , Hines John , Kudahetti Sakunthala , Oliver Tim , Powles Thomas , Alifrangis Constantine , Kohli Manish , Shaw Greg , Wang Wen , Feng Ninghan , Shamash Jonathan , Berney Daniel , Wang Liang , Lu Yong-Jie TITLE=The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=8 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.602493 DOI=10.3389/fcell.2020.602493 ISSN=2296-634X ABSTRACT=
Castration-resistant prostate cancer (CRPC) is the major cause of death from prostate cancer. Biomarkers to improve early detection and prediction of CRPC especially using non-invasive liquid biopsies could improve outcomes. Therefore, we investigated the plasma exosomal miRNAs associated with CRPC and their potential for development into non-invasive early detection biomarkers for resistance to treatment. RNA-sequencing, which generated approximately five million reads per patient, was performed to identify differentially expressed plasma exosomal miRNAs in 24 treatment-naive prostate cancer and 24 CRPC patients. RT-qPCR was used to confirm the differential expressions of six exosomal miRNAs, miR-423-3p, miR-320a, miR-99a-5p, miR-320d, miR-320b, and miR-150-5p (