Ya-Nan Wang
Hong-Zhong Jin- Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
N6-methyladenosine (m6A) methylation, as the most prevalent internal RNA modification, has been revealed to play critical roles in various biological functions. In this study, we performed m6A transcriptome-wide profiling in three kinds of skin tissue: involved psoriatic skin (PP), uninvolved psoriatic skin (PN), and healthy control skin samples (NN). The findings revealed that transcripts of PP contained the fewest m6A peaks and lowest m6A peak density. The greatest differences of m6A methylation were observed in the PP vs. NN and PP vs. PN comparisons. Intriguingly, in these comparisons, hypermethylated m6A was mainly enriched within the CDSs and 3′UTRs, while hypomethylated m6A was not only enriched within CDSs and 3′UTRs, but also within 5′UTRs. GO and KEGG pathway analyses indicated that hypermethylated transcripts in PP were particularly associated with response-associated terms, cytokine production, and olfactory transduction. Meanwhile, hypomethylated transcripts in PP were mainly associated with development-related processes and the Wnt signaling pathway. In addition, we discovered that 19.3–48.4% of the differentially expressed transcripts in psoriasis vulgaris were modified by m6A, and that transcripts with lower expression were more preferentially modified by m6A. Moreover, upregulation of gene expression was often accompanied by upregulation of m6A methylation, suggesting a regulatory role of m6A in psoriasis vulgaris gene expression.
Introduction
Psoriasis is a chronic, systemic, inflammatory disease, affecting about 2% of the world’s population (Greb et al., 2016). The most common subtype is psoriasis vulgaris, accounting for 80–90% of all cases (Greb et al., 2016). In 2014, the WHO adopted a resolution that defines psoriasis as a chronic, non-communicable, painful, disfiguring, and disabling disease for which there is no cure (World Health Organization, 2014). The skin lesions of psoriasis vulgaris are characterized by excessive proliferation and abnormal differentiation of keratinocytes, accompanied by significant infiltration of inflammatory cells, which originate as a result of dysregulation of immunity triggered by environmental and genetic stimuli (Boehncke and Schon, 2015; Hugh and Weinberg, 2018). Situated between heredity and the environment are epigenetic markers, which are another layer of biological information (Pollock et al., 2017). Extensive epidemiological and molecular analyses have provided evidence for the involvement of epigenetics in psoriasis vulgaris (Pollock et al., 2017; Rendon and Schäkel, 2019). The effects of pharmacological inhibitors of epigenetic-modifying enzymes in psoriasis vulgaris have been studied and verified (Bovenschen et al., 2011; Hammitzsch et al., 2015). Epigenetic mechanisms modify gene expression without changing the sequence of the genome, such as long non-coding RNA (lncRNA) and microRNA (miRNA) silencing, and DNA methylation, but RNA modifications in psoriasis vulgaris have not yet been reported (Rendon and Schäkel, 2019).
In the past, the study of epigenetic changes in human diseases mainly focused on DNA methylation and histone modification. Although post-transcriptional modifications of RNA have been known for more than 50 years, the effects of these modifications on the regulation of gene expression has only begun to be explored in recent years due to the previous lack of sensitive detection techniques (He, 2010). Against the background of the rapid development of second-generation sequencing technology, as early as 2012, the sequencing technology of methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-Seq) was developed (Dominissini et al., 2013). m6A (N6-methyladenosine), involving methylation at the 6th position nitrogen atom of adenine (A) of RNA, is the most prevalent RNA modification in messenger RNA (mRNA) and lncRNA of higher eukaryotes (Wang et al., 2017b; Wang et al., 2020). m6A methylation is highly conserved and occurs widely in eukaryotic species ranging from yeast, plant, and drosophila to mammals, as well as viruses (Bi et al., 2019). m6A methylation can be “written” by methyltransferases [e.g., methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), and Wilms tumor 1-associated protein (WTAP)] and “erased” by demethylases [e.g., fat-mass and obesity-associated protein (FTO) and alkB homolog 5 (ALKBH5)], which confirms that m6A methylation is dynamic and reversible (Bi et al., 2019). In addition, the discovery of binding proteins [e.g., YTH domain family 1–3 (YTHDF1–3)] confirmed that m6A methylation has a wide range of biological effects and significance (Fu et al., 2014). In recent years, m6A methylation has emerged as a critical post-transcriptional regulator of gene expression programs, and it can regulate various aspects of RNA, including splicing, transport, translation, and stability (Fu et al., 2014; Frye et al., 2018). It has been found that m6A methylation is closely related to metabolism, carcinogenesis, nervous system, mental, oral, and immune diseases (Church et al., 2010; Engel and Chen, 2018; Pan et al., 2018). However, to the best of our knowledge, no reports have been published on m6A methylation in the context of psoriasis vulgaris.
In this study, we established a transcriptome-wide m6A methylome profile of psoriasis vulgaris, as assessed by MeRIP-Seq. In addition, we used RNA-Seq data to perform a combined analysis of m6A methylation and mRNA levels.
Materials and Methods
Patient Samples
Four patients with psoriasis vulgaris were recruited from the outpatient clinics of the Peking Union Medical College Hospital (Supplementary Table 1 and Supplementary Figure 1). Prior to recruitment, none of the patients had received systemic tretinoin, glucocorticoid, immunosuppressant, or biological agents, nor had they received PUVA/solarium/UV treatment for at least 3 months or topical therapy for at least 2 weeks before the study start. Infectious diseases, tumors, autoimmune diseases, and other immune-related diseases were ruled out. The subjects had no coagulatory disorders or other diseases making them unsuitable to undergo a surgical operation. Skin biopsies were obtained from the patients with psoriasis vulgaris by minimally invasive surgery under aseptic conditions with local anesthesia (0.5% lidocaine). Two types of skin sample were collected from each patient: one from active lesions and the other from skin not exhibiting any of the macroscopic changes related to psoriatic lesions, at least 3 cm away from the active lesions. Four age- and sex-matched healthy controls without a personal or family history of psoriasis vulgaris were enrolled from the Department of Plastic Surgery at Peking Union Medical College Hospital. Healthy skin tissues were obtained from these volunteers. This study was approved by the Ethics Committee of Peking Union Medical College Hospital and informed consent was obtained from all patients and unaffected individuals.
MeRIP-Seq, RNA-Seq, and Data Analysis
The MeRIP-Seq was performed by Cloudseq Biotech Inc. (Shanghai, China), in accordance with the published procedure with slight modifications (Meyer et al., 2012). Briefly, four biological replicates were used for the PP, PN, and NN groups. Total RNA was extracted from the three groups of skin tissue using TRIzol reagent (Life Technologies, Carlsbad, CA, United States). The quality and quantity of total RNA were assessed using NanoDrop ND-2000 (Thermo Fisher Scientific, Waltham, MA, United States). The RNA integrity was measured using denaturing agarose gel electrophoresis. Seq-StarTM poly(A) mRNA Isolation Kit (Arraystar, Rockville, MD, United States) was used to isolate mRNA from total RNA. mRNA was randomly fragmented to 200 nt by RNA Fragmentation Reagents (Ambion) (Invitrogen, Carlsbad, CA, United States). A total of 5 μg of fragmented mRNA was saved as input control for RNA-Seq, while 500 μg of fragmented mRNA was used to perform m6A RNA immunoprecipitation with GenSeqTM m6A-MeRIP Kit (GenSeq, Beijing, China) [including anti-m6A polyclonal antibody (Synaptic Systems, Goettingen, Germany)]. Both the m6A IP sample and the input sample (without immunoprecipitation) were used for library preparation with NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, United States). The library quality was evaluated with BioAnalyzer 2100 system (Agilent Technologies, Santa Clara, CA, United States). Library sequencing was performed on an Illumina HiSeq instrument with 150 bp paired-end reads.
Paired-end reads were harvested from the Illumina HiSeq 4000 sequencer and were subjected to quality control by Q30. After 3′ adaptor-trimming and the removal of low-quality reads by cutadapt software (v1.9.3) (Martin, 2011), the reads were aligned to the reference genome (UCSC HG19) with Hisat2 software (v2.0.4) (Kim et al., 2015). Methylated sites on RNAs (peaks) were identified using Model-based Analysis of ChIP-Seq (MACS) software (Zhang et al., 2008). Peak numbers refer to peaks present in all four samples in each group. Differentially methylated sites (fold change > 2 and P < 0.00001) on mRNAs were identified by diffReps (Shen et al., 2013). Genes of interest were visualized in the IGV (Integrative Genomics Viewer) software (v2.3.68) (Thorvaldsdóttir et al., 2013). Those peaks identified by both software programs overlapping with exons of mRNA were determined and selected using custom-made scripts. The GO analysis and pathway enrichment analysis were performed on the differentially methylated protein-coding genes by using GO1 and KEGG databases2. Sequence motifs were identified using Homer (Heinz et al., 2010). Gene expression was calculated by Cufflinks (Trapnell et al., 2012) using sequencing reads from input samples. Cuffdiff (Trapnell et al., 2012) was used to find DE genes.
MeRIP-RT-qPCR
RNA fragmentation and m6A-immunoprecipitation were performed as described above. Briefly, total RNA was extracted using TRIzol (Life Technologies) and fragmented by RNA fragmentation reagents (Thermo Fisher Scientific) or not. After saving 50 ng of the total RNA as input, the remaining RNA (2 μg) was used for m6A-immunoprecipitation with anti-m6A polyclonal antibody (Synaptic Systems) in 500 μL of IP buffer (150 mM NaCl, 0.1% NP-40, 10 mM Tris, pH 7.4, 100 U RNase inhibitor) to obtain the m6A pull-down portion (m6A IP portion). m6A RNAs were immunoprecipitated with Dynabeads® Protein A (Thermo Fisher Scientific) and eluted twice with elution buffer (5 mM Tris-HCL pH 7.5, 1 mM EDTA pH 8.0, 0.05% SDS, 20 mg/ml Proteinase K). m6A IP RNAs were recovered by ethanol precipitation and RNA concentration was measured with Qubit® RNA HS Assay Kit (Thermo Fisher Scientific). Then, 2 ng of the total RNA and m6A IP RNA were used to synthesize complementary DNA by using the iScript cDNA Synthesis Kit (Bio-Rad, CA, United States). Real-time PCR was subsequently performed, using an SYBR Premix Ex Taq (Takara, Liaoning, China) and ABI 7500 Sequence Detection System (Thermo Fisher Scientific). All procedures were performed in accordance with the manufacturer’s protocols. The sequences of the primers used are presented in Supplementary Table 2.
Statistical Analyses
Experiments were performed at least three times and representative results are shown. Statistical analysis was performed using GraphPad Prism Version 8.0 software. Differences between individual groups were analyzed using the chi-squared test and Student’s t-test (two-tailed and unpaired) with triplicate or quadruplicate sets. Pearson’s correlation was adopted to carry out the correlation analysis. A value of P < 0.05 was considered statistically significant.
Results
Overview of m6A Methylation in Psoriasis Vulgaris mRNA
To obtain the transcriptome-wide m6A map of psoriasis vulgaris, we examined three kinds of skin tissue, namely, involved psoriatic skin (PP), uninvolved psoriatic skin (PN), and healthy control skin samples (NN), using m6A-targeted antibody coupled with high-throughput sequencing (i.e., MeRIP-Seq). Psoriasis Area and Severity Index (PASI) scores for psoriatic patients ranged from 10.8 to 16.7. Using Illumina HiSeq 4000, we acquired 68,678,097, 62,087,334, and 62,121,508 reads from PP, PN, and NN, respectively. After end-trimming and quality filtering, 57,334,158, 45,130,912, and 49,749,336 high-quality reads (83.11, 72.65, and 80.04% of the total reads) from PP, PN, and NN, respectively, were mapped to the human reference genome (UCSC HG19) (Supplementary Table 3).
The fewest m6A peaks (sites) were identified in PP, while the most were identified in PN. Specifically, 16,868 m6A peaks were identified from 16,520 genes in PP, 22,144 m6A peaks were identified from 17,665 genes in PN, and 20,408 m6A peaks were identified from 17,358 genes in NN (Figure 1A, Supplementary Figure 2, Supplementary Table 4, and Supplementary Data 1). We next compared the shared peaks between groups; we found that transcripts of PP and NN carried the fewest shared m6A peaks (a total of 15,176 m6A peaks from 10,604 genes), while transcripts of PN and NN carried the largest number of shared m6A peaks (a total of 17,678 m6A peaks from 11,816 genes), suggesting that the difference between PP and NN was greater than that between PN and NN (P < 0.001) (Figure 1B).
Figure 1. Overview of m6A methylome in psoriatic vulgaris skin. (A) Comparison of the number of m6A peaks identified in PP and PN. (B) Numbers of group-specific and common m6A peaks. (C) Estimation of m6A density on transcripts. Transcripts with different expression level are divided to 10 groups, and the m6A density of PP, PN, and NN samples is calculated separately. (D) Percentage of m6A methylated genes with different m6A peak number. (E) The top five motifs enriched across m6A peaks identified from involved psoriatic skin samples. (F) Distribution of m6A peaks across the length of mRNAs. 5′UTRs, CDS, and 3′UTRs of PP mRNAs are individually binned into regions spanning 1% of their total length, and the percentage of m6A peaks that fall within each bin is determined. (G) The distribution of m6A peak from PP along a metagene.
m6A Peak Density and Distribution Pattern in Psoriasis Vulgaris mRNA
Based on the above results, we estimated that there were 0.62–0.75 m6A peaks per 1,000 nucleotides or 1.02–1.25 m6A peaks per identified transcript in PP, PN, and NN. Among them, transcripts of PP contained the lowest m6A peak density (0.62/1k nt or 1.02/gene), while transcripts of PN contained the highest (0.75/1k nt or 1.25/gene) (Figure 1C and Supplementary Table 4). The numbers of m6A peaks varied widely among individual genes, and most of them contained a single m6A peak. Specifically, 59.68, 52.95, and 55.25% of the methylated transcripts contained a single m6A peak in PP, PN, and NN, while 26.07, 28.42, and 27.15% of the methylated transcripts contained two m6A peaks per mRNA, respectively. For three m6A peaks, the percentages were further reduced to 8.88, 11.13, and 10.46% in PP, PN, and NN, respectively. Only 5.38, 7.50, and 7.14% of the methylated transcripts containing more than three m6A peaks (Figure 1D). To determine whether the identified m6A peaks were enriched at consensus sequences of RRACH (R represents purine, A is m6A, and H is a non-guanine base), we performed motif analysis and found consistent results in PP, PN, and NN (Figure 1E and Supplementary Figure 3). To analyze the distribution pattern of m6A in psoriasis vulgaris, the metagene profiles of all identified m6A peaks were investigated in the transcriptomes of PP, PN, and NN, which revealed that m6A peaks were highly enriched within the coding sequences (CDSs) and 3′ untranslated regions (3′UTRs) (Figures 1F,G and Supplementary Figure 4). These features of m6A methylation indicate its high conservation in psoriasis vulgaris.
Analysis of Differentially Methylated RNAs Among PP, PN, and NN Samples
We next analyzed the differentially methylated RNAs (DMRs). The greatest differences were observed in the PP vs. NN and PP vs. PN comparisons (Figure 2A, Supplementary Table 5, and Supplementary Data 2). In the PP vs. NN and PP vs. PN comparisons, the number of hypomethylated m6A peaks was much greater than that of hypermethylated m6A peaks in PP (P < 0.001). Specifically, compared with NN, transcripts of PP contained more hypomethylated m6A peaks (1,719 m6A peaks from 1,113 genes) than hypermethylated ones (1,470 m6A peaks from 1,127 genes). When compared with PN, transcripts of PP also contained more hypomethylated m6A peaks (2,316 m6A peaks from 1,568 genes) than hypermethylated ones (2,024 m6A peaks from 1,362 genes) (Figure 2A and Supplementary Table 5). However, when compared with NN, PN contained slightly more hypermethylated m6A peaks (914 m6A peaks from 691 genes) than hypomethylated ones (755 m6A peaks from 537 genes), but this difference was relatively small compared with those of PP vs. NN and PP vs. PN (P < 0.001) (Figure 2A and Supplementary Table 5). Furthermore, upon analyzing the m6A peak distribution pattern of these DMRs in PP vs. NN, PP vs. PN, and PN vs. NN, we found that the hypermethylated m6A peaks were mainly enriched within CDSs and 3′UTRs, while hypomethylated m6A peaks were not only enriched within CDSs and 3′UTRs, but also highly enriched within 5′UTRs (Table 1).
Figure 2. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses of coding genes containing altered m6A peaks. (A) Comparison of the number of hypermethylated and hypomethylated m6A peaks identified in PP, PN, and NN samples. (B) The top 10 gene ontology terms were significantly enriched for the hypermethylated genes in PP vs. NN. (C) The top 10 gene ontology terms were significantly enriched for the hypomethylated genes in PP vs. NN. (D) The top 10 significantly enriched pathways for the hypermethylated genes in PP vs. NN. (E) The top 10 significantly enriched pathways for the hypomethylated genes in PP vs. NN.
Table 1. Number of m6A peaks among PP, PN, and NN samples.
Gene Ontology Analysis of Differentially Methylated RNAs Among PP, PN, and NN Samples
To deduce the potential biological significance of m6A methylation in psoriasis vulgaris, we analyzed these DMRs by performing Gene Ontology (GO) analysis. GO analysis revealed that, compared with PN or NN, hypermethylated DMRs in PP were particularly associated with response-related items [e.g., defense response, response to other organisms, inflammatory response, and response to (external) biotic stimulus] and cytokine-related items (e.g., regulation of cytokine production, cytokine production, and interleukin-6 production), suggesting that these hypermethylated DMRs may be involved in the response to environmental stimulations at the initial stage of psoriasis vulgaris (Figure 2B, Supplementary Figure 5A, and Supplementary Data 3). Meanwhile, hypomethylated DMRs in PP were mainly enriched in development-related items (e.g., multicellular organism development, system development, anatomical structure development, developmental process, and nervous system development) and cell–cell signaling, which suggested that the function of hypomethylated DMRs differed from that of hypermethylated DMRs. Hypomethylated DMRs in PP were mainly focused on cell and tissue development processes (Figure 2C, Supplementary Figure 5B, and Supplementary Data 3). In addition to these shared gene ontological terms, DMRs in the PP vs. NN and PP vs. PN comparisons were also associated with some specific terms. For example, in PP vs. NN, hypermethylated DMRs in PP were specifically associated with the G-protein-coupled receptor signaling pathway (Figure 2B and Supplementary Data 3), including cannabinoid receptors that are involved in the proliferation/differentiation and immune activity of keratinocytes, indicating that these hypermethylated DMRs may participate in critical processes of psoriasis vulgaris (Tóth et al., 2019). Meanwhile, hypomethylated DMRs in PP were specifically associated with the polyol metabolic process (Figure 2C and Supplementary Data 3), which may be related to the previous epidemiological finding that psoriasis patients tended to suffer from metabolic diseases (Hu et al., 2019). When compared with PN, hypermethylated DMRs in PP were specifically associated with cell cycle-related items (e.g., mitotic cell cycle process, mitotic cell cycle, cell cycle, and mitotic nuclear division) (Supplementary Figure 5A and Supplementary Data 3), while hypomethylated DMRs in PP were specifically associated with negative regulation of cellular component movement, locomotion, and learning (Supplementary Figure 5B and Supplementary Data 3). Given that the cell cycle-related genes were dysregulated in keratinocytes in psoriasis vulgaris, m6A methylation should play an important role in dysregulation of the cell cycle in keratinocytes in psoriasis vulgaris (Pasquali et al., 2019). Besides, PN and NN exhibited various differences, and the DMRs were mainly associated with system process and multicellular organismal process (Supplementary Figures 5C,D and Supplementary Data 3). All of these results suggest that m6A methylation participates in various pathophysiological aspects of psoriasis vulgaris.
Kyoto Encyclopedia of Genes and Genomes Pathway Analysis of Differentially Methylated Genes Among PP, PN, and NN Samples
Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DMRs was also conducted for both hypermethylated and hypomethylated genes. This analysis showed that, compared with NN or PN, hypermethylated DMRs in PP were mainly associated with cytokine–cytokine receptor interaction (Figure 2D, Supplementary Figure 5E, and Supplementary Data 4); hypomethylated DMRs in PP were mainly associated with the Wnt signaling pathway (Figure 2E, Supplementary Figure 5F, and Supplementary Data 4). We used quantitative reverse-transcription PCR (RT-qPCR) to validate the important genes WNT5A, DIF1, and DKK2 in Wnt signaling pathway, as well as TNF, IL17A, HIF1A, and SOCS1/3, which are associated with the pathophysiology of psoriasis; all of them showed significant enrichment in immunoprecipitation (IP) pull-down samples (Figure 3 and Supplementary Figure 6). In addition to these shared pathways, DMRs in the PP vs. NN and PP vs. PN comparisons were also associated with some specific pathways. For example, in PP vs. NN, hypermethylated DMRs in PP were specifically associated with olfactory transduction (Figure 2D and Supplementary Data 4), which may explain why psoriasis patients have an abnormal sense of smell (Aydın et al., 2016); meanwhile, hypomethylated DMRs in PP were specifically associated with the MAPK signaling pathway (Figure 2E and Supplementary Data 4), which is involved in almost every aspect of psoriasis vulgaris, including keratinocyte proliferation, differentiation, migration, T-helper-cell differentiation, and angiogenesis (Mavropoulos et al., 2013). In the PP vs. PN comparison, DMRs were specifically associated with the cell cycle and gap junction (Supplementary Figures 5E,F and Supplementary Data 4). Besides, from the comparison with NN, DMRs in PN were mainly associated with vascular smooth muscle contraction and osteoclast differentiation (Supplementary Figures 5G,H and Supplementary Data 4).
Figure 3. Examples of genes with m6A peaks in PP, PN, and NN. Integrative Genomics Viewer (IGV) tracks showed MeRIP-seq reads distribution in TNF, IL-17A, SOCS1/3, HIF1A, WNT5A, WIF1, DKK2, CNR2, and GPR55 mRNA of PP, PN, and NN. Different colors illustrate the accumulation of m6A-IP reads. The blue squares indicate the verified sequence fragments.
Relationship Between m6A Peaks and mRNA Level
We next performed MeRIP-Seq and RNA-Seq combined analysis to explore whether the extent of m6A methylation was associated with the mRNA level of the differentially expressed genes (DEGs). Specifically, upon comparison with NN, 511 highly expressed DEGs were identified in PP, of which 116 (22.7%) transcripts were modified by m6A, 87.9% (102/116) of which were hypermethylated. Meanwhile, 773 transcripts expressed at lower levels were identified in PP, of which 340 (44.0%) were modified by m6A, 97.6% (332/340) of which were hypomethylated (Table 2, Figure 4A, and Supplementary Data 5). The comparison of PP vs. PN and PN vs. NN showed a similar pattern to the comparisons of PP vs. NN (Table 2, Supplementary Figures 7A,B, and Supplementary Data 5). Based on these results, we estimated that 19.3–48.4% of the DEGs (transcripts) were chemically modified by m6A. In the PP vs. NN and PP vs. PN comparisons, the proportion of transcripts expressed at lower levels that were modified by m6A was higher than that of highly expressed transcripts (46.1 vs. 23.7%, P < 0.001), suggesting that transcripts expressed at lower levels were more preferentially modified by m6A. These results also showed that the upregulation of gene expression was often accompanied by the upregulation of m6A methylation (r = 1, P < 0.05). Furthermore, we classified these m6A-containing DEGs by their peak position and examined how their mRNA expression levels correlated with the locations of m6A peaks. Our analysis showed that, irrespective of whether the m6A peaks were located at a 5′UTR, CDS, intron, or 3′UTR, their extent of methylation was always positively correlated with the mRNA expression levels (P < 0.001) (Figure 4B, Supplementary Figure 8, and Supplementary Data 6).
Table 2. Relationship between m6A peaks and mRNA level.
Figure 4. Relationship between m6A peaks and mRNA level. (A) Differentially expressed genes (transcripts) in PP and NN. Genes contained up-regulated m6A peaks are highlighted in red and genes contained down- regulated m6A peaks are highlighted in blue. (B) The ratio of gene expression levels in PP and NN samples containing up-regulated m6A peaks. Genes are divided into four categories (5′UTR, CDS, Intonic, 3′UTR) according to the peak positions. (C) Cumulative distribution of mRNA expression changes between PP and NN for m6A -modified genes (red) and non-target genes (green). P-values are calculated by two-sided Mann–Whitney test.
To compare the mRNA expression levels of methylated and unmethylated transcripts, we divided the whole transcriptome genes according to whether they were modified by m6A. However, we found that, in the PP vs. NN comparison, among m6A methylated genes, more were upregulated than downregulated; among non-m6A methylated genes, more were downregulated than upregulated. This trend was consistent with the PP vs. PN and PN vs. NN comparisons (Figure 4C and Supplementary Figure 9).
Discussion
Extensive m6A-related studies have been performed in malignant tumors, nervous system diseases, hematopoietic diseases, metabolic diseases, viral diseases, and injuries, but no reports have been published on m6A profiling in patients with psoriasis vulgaris (Brocard et al., 2017; Zhang et al., 2017; Sun et al., 2019). In this study, two sequencing libraries, namely, m6A-Seq library (IP) and RNA-Seq library (input), were constructed for high-throughput MeRIP-Seq to examine transcriptome-wide m6A methylation patterns in a total of 12 skin samples, four each from PP, PN, and NN biopsies. Our data showed that m6A is highly conserved across psoriasis vulgaris and healthy controls. Nevertheless, there are differences among PP, PN, and NN, with ∼5,624 fewer m6A peaks identified in PP samples than in PN samples.
m6A-modified nucleotides were previously shown to be widely distributed in human tissues including the liver, kidney, brain, lung, and heart (Dominissini et al., 2012; He et al., 2019; Lan et al., 2019; Mathiyalagan et al., 2019). Here, we detected 1.021, 1.254, and 1.176 m6A peaks per gene in skin samples of PP, PN, and NN, respectively. These results further affirm that m6A is a universal form of RNA modification in human tissues. However, the levels of m6A methylation in skin samples of PP, PN, and NN were lower than the previous estimations of approximately ∼1.7 m6A residues per gene in a human hepatocellular carcinoma cell line (HepG2) and ∼3 m6A residues per average mRNA transcript in mammalian cells (Dominissini et al., 2012). The differences in proportions of m6A-modified transcripts may be due to the different tissue types. The pattern of adenosine methylation in mRNAs of PP, PN, and NN is consistent with that reported in mouse brain, in that a few of the m6A peaks are clustered together while most of them are single (Meyer et al., 2012). However, the proportions of single peaks of PP, PN, and NN (59.68, 52.95, and 55.25%) are even higher than that of mouse brain (46%) (Meyer et al., 2012). We assumed that this difference might be associated with the different tissue types and gene structure characteristics.
Comparative analysis among groups showed that PP and NN contained the fewest shared m6A peaks, while PN and NN carried the most. In addition, DMR analysis revealed that the greatest differences were observed in the PP vs. NN and PP vs. PN comparisons, instead of PN and NN, suggesting that the difference in m6A methylation between PP and NN was greater than that between PN and NN. Compared with PN or NN, the number of hypomethylated DMRs in PP was much greater than that of hypermethylated DMRs, suggesting that DMRs in PP were preferentially hypomethylated. These hypermethylated m6A peaks in PP were mainly enriched in CDSs and 3′UTRs, which is consistent with previous studies (Meyer et al., 2012). However, the hypomethylated m6A peaks in PP were not only enriched in CDSs and 3′UTRs, but also highly enriched in 5′UTRs. Because 5′UTRs play a major role in controlling translation efficiency and shaping the cellular proteome (Hinnebusch et al., 2016), we assume that the hypomethylated DMRs in PP may be involved in protein translation and shaping.
GO analysis revealed that, compared with PN or NN, hypermethylated DMRs in PP were mainly linked to response-associated terms (e.g., response to other organisms, inflammatory response, and response to external/biotic stimulus) and cytokine production and regulation. Because psoriasis is often triggered by internal or external environmental stimuli and persists due to cross-talk between keratinocytes and immunocytes, which mediates the production of cytokines, chemokines, and growth factors (Hugh and Weinberg, 2018), it is suggested that m6A methylation may be involved in the important pathogenic processes of psoriasis, including triggering the disease and maintaining inflammation. Distinct from hypermethylated DMRs in PP, the hypomethylated DMRs in PP were mainly associated with development-related terms, suggesting that hypomethylated DMRs in PP may participate in development-related processes. In addition, compared with NN, highly methylated DMRs in PP were specifically enriched in the G-protein-coupled receptor signaling pathway. Studies have reported that cannabinoid type 2 receptor (CB2) and G-protein-coupled receptor 55 (GPR55), as G-protein-coupled receptors, were both increased in psoriasis vulgaris and could attenuate oxidative stress and act in an anti-inflammatory process (Ambrożewicz et al., 2018; Figure 3). Another study reported that cannabinoid receptors control the proliferation/differentiation and immune activity of keratinocytes (Tóth et al., 2019). While both CB2 and GPR55 were modified by m6A, there was no significant difference among PP, PN and NN in our study (Figure 3). Meanwhile, hypomethylated DMRs in PP were particularly associated with the polyol metabolic process, which is one of the major biochemical pathways involved in the development of diabetic macroangiopathy and peripheral neuropathy (Katakami, 2018). It has been shown that there is a significant correlation between psoriasis/diabetes and the polyol metabolic process (Mamizadeh et al., 2019), so we consider that these hypomethylated DMRs in PP may provide a clue to explain why psoriasis is related to diabetes.
Interestingly, KEGG pathway analysis revealed that, compared with NN, highly methylated DMRs in PP were mainly associated with olfactory receptor genes. Ahn et al. (2016) reported that modules linked to psoriasis were mainly associated with olfactory signaling, as assessed by RNA-Seq. Olfactory receptors are known to be expressed not only in nasal tissue but also in skin tissue and specifically in keratinocytes, dendritic cells, and melanocytes (Ahn et al., 2016). In addition, Aydın et al. (2016) found that the olfactory function of patients with psoriasis was significantly worse than that of healthy controls (P < 0.001). These results suggest that m6A methylation may be involved in the mechanism of anosmia in psoriasis vulgaris. In addition, our studies showed that hypomethylated transcripts in PP were mainly associated with the Wnt signaling pathway. The Wnt gene family is known to encode a set of highly conserved secreted signaling proteins that participate in and control cell differentiation, cell proliferation, and immune-mediated inflammatory cascade in psoriasis vulgaris (Gudjonsson et al., 2010; Dou et al., 2017; Wang et al., 2017a). This suggests that m6A methylation may control the critical pathogenic processes in psoriasis vulgaris, including cell differentiation, cell proliferation, and immune-mediated inflammation, by modifying expression of the Wnt gene family (Irrera et al., 2020). Besides, we found that IL-17A and TNF-α, two of the key genes of the TNF-α/IL-23/Th17 axis in psoriasis, are upregulated > 20-fold and > 3.5-fold in m6A methylation levels in PP vs. NN, respectively (Figure 3). This axis is widely regarded as the core process of the pathogenesis of psoriasis vulgaris, which could induce the proliferation of keratinocytes and form the inflammatory plaque of psoriasis vulgaris (Rendon and Schäkel, 2019). The rapid and efficient therapeutic response of various monoclonal antibodies against TNF-α or IL-17A strongly supports the TNF-α/IL-23/Th17 axis as being a key factor for the expansion of inflammation and the aggravation of skin lesions in psoriasis vulgaris (Rendon and Schäkel, 2019). Therefore, these findings support the notion that m6A may be a critical denominator that controls keratinocyte proliferation, cell differentiation, and inflammation in psoriasis vulgaris.
RNA-Seq data were used for the combined analysis of m6A peaks and mRNA level. Upon comparison with PN or NN, about 20% of the highly expressed genes in PP (PP-high) were chemically modified by m6A, while about 40% of the genes expressed at lower levels in PP (PP-low) were chemically modified by m6A, which was close to the findings in a previous report (over one-third) on the human brain (Dominissini et al., 2012). The estimated difference between PP-high and PP-low suggested that genes expressed at lower levels were preferentially modified by m6A. We extended this analysis to the whole transcriptome. However, among genes expressed at a low level in PP, the proportion of m6A targets was smaller than that of non-m6A targets; meanwhile, among highly expressed genes in PP, the proportion of m6A targets was greater than that of non-m6A targets. The reason for the results being opposite between the two analyses may be the different data ranges. The former analysis only analyzed DEGs, while the latter analysis analyzed the whole transcriptome. In previous studies on A. thaliana, most transcripts with a low expression level were more likely modified by m6A in both leaf and flower chloroplasts (Wan et al., 2015; Wang et al., 2017c). However, the root amyloplast presented the methylation feature that the moderately expressed transcripts were more likely to be methylated, and those expressed at the two extremes were less methylated by m6A (Wan et al., 2015; Wang et al., 2017c). These results indicated that the relationship between the level of mRNA and whether there is m6A modification differs among different species, organs and tissues. Furthermore, we analyzed the relationship between m6A level and mRNA level, and found that the upregulation of gene expression was often accompanied by the upregulation of m6A methylation regardless of the peak position, suggesting a possible positive relationship between the extent of m6A methylation and the mRNA levels. Actually, many researches have revealed that m6A level was positively or negatively correlated with mRNA level (Zhao et al., 2017). Mostly, m6A methylation was shown to be negatively correlated with mRNA level when m6A methylation was linked to accelerate degradation of target transcripts (Liu et al., 2014). For example, silencing of m6A writers (METTL3, METTL14, or WTAP) in mammalian cells has been shown to lead to increases in the abundance of their respective target transcripts (Liu et al., 2014). Overexpression of m6A reader (YTHDF2) in human embryonic kidney 293T cells has been shown to lead to decreases in the abundance of the target transcripts (Wu et al., 2020). Besides, m6A methylation was shown to be positively correlated with mRNA level when m6A methylation was linked to enhance mRNA expression (Zhao et al., 2017). For example, METTL3-mediated m6A methylation enhanced ZMYM1 mRNA expression in gastric cancer (Yue et al., 2019). The role of m6A methylation in transcriptional regulation needs to be elucidated by further studies in the future.
Data Availability Statement
The datasets generated for this study can be found in the online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE155702.
Ethics Statement
The studies involving human participants were reviewed and approved by the Ethics Committee of Peking Union Medical College Hospital. The patients/participants provided their written informed consent to participate in this study.
Author Contributions
Y-NW conceived the project, performed the experiments, performed data analyses, and wrote the manuscript. H-ZJ reviewed the manuscript and made substantial contributions to the drafting process. All authors agreed to be accountable for the content of the work.
Funding
This study was funded by the National Natural Science Foundation of China (82073450), the National Key Research and Development Program of China (No. 2016YFC0901500), and the Chinese Academy of Medical Sciences (CAMS) Initiative for Innovative Medicine (2017-I2M-B&R-01).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2020.591629/full#supplementary-material
Footnotes
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Keywords: N6-methyladenosine, m6A methylation, psoriasis vulgaris, MeRIP-seq, RNA modification
Citation: Wang Y-N and Jin H-Z (2020) Transcriptome-Wide m6A Methylation in Skin Lesions From Patients With Psoriasis Vulgaris. Front. Cell Dev. Biol. 8:591629. doi: 10.3389/fcell.2020.591629
Received: 05 August 2020; Accepted: 14 October 2020;
Published: 05 November 2020.
Edited by:
Giovanni Nigita, The Ohio State University, United StatesReviewed by:
Natalia Pinello, Royal Prince Alfred Hospital, AustraliaHaobo Li, Harvard Medical School, United States
Copyright © 2020 Wang and Jin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Hong-Zhong Jin, jinhongzhong@263.net