AUTHOR=Taylor Richard W. , Holler Cornelia , Mahmoodabadi Reza Gholami , Küppers Michelle , Dastjerdi Houman Mirzaalian , Zaburdaev Vasily , Schambony Alexandra , Sandoghdar Vahid TITLE=High-Precision Protein-Tracking With Interferometric Scattering Microscopy JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=8 YEAR=2020 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.590158 DOI=10.3389/fcell.2020.590158 ISSN=2296-634X ABSTRACT=

The mobility of proteins and lipids within the cell, sculpted oftentimes by the organization of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behavior. Interferometric scattering (iSCAT) microscopy is an emerging technique well-suited for visualizing the diffusion of gold nanoparticle-labeled membrane proteins to a spatial and temporal resolution beyond the means of traditional fluorescent labels. We discuss the applicability of interferometric single particle tracking (iSPT) microscopy to investigate the minutia in the motion of a protein through measurements visualizing the mobility of the epidermal growth factor receptor in various biological scenarios on the live cell.