AUTHOR=Qiu Jin , Zhang Zhiyin , Wang Sainan , Chen Yanru , Liu Caizhi , Xu Sainan , Wang Dongmei , Su Junlei , Ni Mengshan , Yu Jian , Cui Xiangdi , Ma Lu , Hu Tianhui , Hu Yepeng , Gu Xuejiang , Ma Xinran , Wang Jiqiu , Xu Lingyan 

TITLE=Transferrin Receptor Functionally Marks Thermogenic Adipocytes

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.572459

DOI=10.3389/fcell.2020.572459

ISSN=2296-634X

ABSTRACT=<sec><title>Background</title><p>Thermogenic adipocytes, including beige and brown adipocytes, are critical for thermogenesis and energy homeostasis. Identification of functional cell surface markers of thermogenic adipocytes is of significance for potential application in biological and clinical practices.</p></sec><sec><title>Methods</title><p>With a combination of RNA-sequencing of <italic>in vivo</italic> and <italic>in vitro</italic> models, we identified transferrin receptor (Tfr1), a receptor specialized for cellular iron uptake, as a previously unappreciated cell surface molecule for thermogenic adipocytes compared to white adipocytes. The alternation of Tfr1 levels under physiological and pathological stimuli was assessed, and the mitochondria functionality, browning capacity, and iron metabolism of mature adipocytes were examined with <italic>Tfr1</italic> knockdown.</p></sec><sec><title>Results</title><p>Tfr1 was expressed predominantly in thermogenic adipocytes versus white adipocyte, and its expression levels were tightly correlated with the activation or inhibition status of thermogenic adipocytes under external stimuli. Besides, <italic>Tfr1</italic> gene deficiency in thermogenic adipocytes led to reduced thermogenic gene programs and mitochondrial integrity.</p></sec><sec><title>Conclusion</title><p>Tfr1 functionally marks thermogenic adipocytes and could serve as a potential thermogenic adipocyte surface marker.</p></sec>