AUTHOR=Tan Shirui , Yan Fang , Li Qingrong , Liang Yaping , Yu Junxu , Li Zhenjun , He Feifei , Li Rongpeng , Li Ming 

TITLE=Chlorogenic Acid Promotes Autophagy and Alleviates Salmonella Typhimurium Infection Through the lncRNAGAS5/miR-23a/PTEN Axis and the p38 MAPK Pathway

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.552020

DOI=10.3389/fcell.2020.552020

ISSN=2296-634X

ABSTRACT=<sec><title>Background</title><p><italic>Salmonella typhimurium (ST</italic>) causes several intestinal diseases. Polyphenols including chlorogenic acid (CGA) inhibit pathogenesis.</p></sec><sec><title>Objective</title><p>This study aimed to investigate the mechanisms of CGA in <italic>ST</italic> infection.</p></sec><sec><title>Methods</title><p>The intestinal pathological changes and survival rate of ST-infected mice were measured to verify the protection of CGA on <italic>ST</italic> infection. The antibacterial effects of CGA <italic>in vitro</italic> on the invasion to intestinal epithelial cells and autophagy was evaluated. The relationships among GAS5, miR-23a, and PTEN were verified. Expression of inflammation- and autophagy-related proteins was detected.</p></sec><sec><title>Results</title><p>CGA treatment alleviated pathological damage, improved the secretion disturbance of intestinal cytokines caused by <italic>ST</italic> infection, and reduced the mortality of mice. Intestinal GAS5 was upregulated after CGA treatment. LncRNA GAS5 competitively bound to miR-23a to upregulate PTEN and inhibit the p38 MAPK pathway. CGA regulated the p38 MAPK pathway through lncRNA GAS5/miR-23a/PTEN axis to promote autophagy in <italic>ST</italic> infection. The functional rescue experiments of miR-23a and PTEN further identified these effects.</p></sec><sec><title>Conclusion</title><p>CGA promotes autophagy and inhibits <italic>ST</italic> infection through the GAS5/miR-23a/PTEN axis and the p38 MAPK pathway.</p></sec>