AUTHOR=Xu Chenze , Mohsin Ali , Luo Yanxia , Xie Lili , Peng Yan , Wang Qizheng , Ahmed Waqas , Hang Haifeng , Zhuang Yingping , Guo Meijin TITLE=Inducing Non-genetically Modified Induced Embryonic Sertoli Cells Derived From Embryonic Stem Cells With Recombinant Protein Factors JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=8 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.533543 DOI=10.3389/fcell.2020.533543 ISSN=2296-634X ABSTRACT=
Embryonic Sertoli cells (eSCs) possess multiple supporting functions and research value in gonadal development and sex determination. However, the limitation of acquiring quality eSCs had hindered the further application. Herein, we successfully derived non-genetically modified (non-GM)-induced embryonic Sertoli-like cells (eSLCs) from mouse embryonic stem cells (ESCs) with a TM4 cell-derived conditioned medium containing recombinant endogenous protein factors