AUTHOR=Peng Hui , Guo Qi , Xiao Ye , Su Tian , Jiang Tie-Jian , Guo Li-Juan , Wang Min 

TITLE=ASPH Regulates Osteogenic Differentiation and Cellular Senescence of BMSCs

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=8

YEAR=2020

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.00872

DOI=10.3389/fcell.2020.00872

ISSN=2296-634X

ABSTRACT=<p>Osteogenesis and senescence of BMSCs play great roles in age-related bone loss. However, the causes of these dysfunctions remain unclear. In this study, we identified a differentially expressed <italic>ASPH</italic> gene in middle-aged and elderly aged groups which were obtained from GSE35955. Subsequent analysis in various databases, such as TCGA, GTEx, and CCLE, revealed that <italic>ASPH</italic> had positive correlations with several osteogenic markers. The depletion of mouse <italic>Asph</italic> suppressed the capacity of osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). Notably, the expression of <italic>ASPH in vitro</italic> decreased during aging and senescence. The deficiency of <italic>Asph</italic> accelerated cellular senescence in BMSCs. Conversely, the overexpression of <italic>Asph</italic> enhanced the capacity of osteogenic differentiation and inhibited cellular senescence. Mechanistically, <italic>ASPH</italic> regulated Wnt signaling mediated by Gsk3β. Taken together, our data established that <italic>ASPH</italic> was potentially involved in the pathogenesis of age-related bone loss through regulating cellular senescence and osteogenic differentiation, which provides some new insights to treat age-related bone loss.</p>