AUTHOR=Kataoka Kensuke , Kurimoto Ryota , Tsutsumi Hiroki , Chiba Tomoki , Kato Tomomi , Shishido Kana , Kato Mariko , Ito Yoshiaki , Cho Yuichiro , Hoshi Osamu , Mimata Ayako , Sakamaki Yuriko , Nakamichi Ryo , Lotz Martin K. , Naruse Keiji , Asahara Hiroshi TITLE=In vitro Neo-Genesis of Tendon/Ligament-Like Tissue by Combination of Mohawk and a Three-Dimensional Cyclic Mechanical Stretch Culture System JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=8 YEAR=2020 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2020.00307 DOI=10.3389/fcell.2020.00307 ISSN=2296-634X ABSTRACT=
Tendons and ligaments are pivotal connective tissues that tightly connect muscle and bone. In this study, we developed a novel approach to generate tendon/ligament-like tissues with a hierarchical structure, by introducing the tendon/ligament-specific transcription factor Mohawk (MKX) into the mesenchymal stem cell (MSC) line C3H10T1/2 cells, and by applying an improved three-dimensional (3D) cyclic mechanical stretch culture system. In our developed protocol, a combination of stable