AUTHOR=Zhou Juan , Lei Jian , Wang Jun , Lian Chen-Lu , Hua Li , He Zhen-Yu , Wu San-Gang 

TITLE=Bioinformatics-Based Discovery of CKLF-Like MARVEL Transmembrane Member 5 as a Novel Biomarker for Breast Cancer

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00361

DOI=10.3389/fcell.2019.00361

ISSN=2296-634X

ABSTRACT=<p>Chemokine-like factor (CKLF)-like <italic>MARVEL</italic> transmembrane members (<italic>CMTMs</italic>) represent a novel protein family linking the chemokine and transmembrane-4 superfamily families, which potentially play several roles in diverse physiological and pathological processes. The detailed functions and underlying molecular mechanisms of <italic>CMTMs</italic> remain elusive in breast cancer. Herein, we performed a comprehensive bioinformatic analysis to investigate the prognostic effect, potential functions, and biomolecular regulatory network of <italic>CMTMs</italic> in breast cancer. The mRNA expression level of <italic>CMTM5</italic>, in particular, was significantly downregulated in breast cancer; moreover, high mRNA expression level of <italic>CMTM5</italic> was significantly associated with better relapse-free survival. DNA promoter hypermethylation of <italic>CMTM5</italic> was negatively correlated with its mRNA expression levels. Furthermore, <italic>CMTM5</italic> strongly associated with pathway in MARVEL domains, chemotaxis, cytokines, transmembrane structures, and integral component of membrane. For example, genes related to MARVEL domains, transmembrane structures, and chemokines were significantly enriched. Our findings indicate that <italic>CMTM5</italic> can be used as a prognostic biomarker and potential therapeutic target for breast cancer.</p>