AUTHOR=Fernández-González Raúl , Laguna Ricardo , Ramos-Ibeas Priscila , Pericuesta Eva , Alcalde-Lopez Víctor , Perez-Cerezales Serafín , Gutierrez-Adan Alfonso TITLE=Successful ICSI in Mice Using Caput Epididymal Spermatozoa JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=7 YEAR=2019 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00346 DOI=10.3389/fcell.2019.00346 ISSN=2296-634X ABSTRACT=
Spermatozoa undergo their last phase of spermiogenesis, known as maturation, as they pass through the epididymis. A recent report indicates that mouse immature spermatozoa retrieved from the caput epididymis for intracytoplasmic sperm injection (ICSI) give rise to embryos with multiple developmental defects. Further, these embryos were unable to develop to term after their transfer to surrogate mothers. Herein, we examined the potential of mouse caput spermatozoa to produce normal embryos by comparing the use of caput vs. cauda epididymal spermatozoa for