AUTHOR=Dolz Mikel , Mateljak Ivan , Méndez-Sánchez Daniel , Sánchez-Moreno Israel , Gomez de Santos Patricia , Viña-Gonzalez Javier , Alcalde Miguel TITLE=Colorimetric High-Throughput Screening Assay to Engineer Fungal Peroxygenases for the Degradation of Thermoset Composite Epoxy Resins JOURNAL=Frontiers in Catalysis VOLUME=2 YEAR=2022 URL=https://www.frontiersin.org/journals/catalysis/articles/10.3389/fctls.2022.883263 DOI=10.3389/fctls.2022.883263 ISSN=2673-7841 ABSTRACT=
At present, the end-of-life management of thermoset composite epoxy resins is limited to incineration and landfill storage, highlighting the demand for the development of more sustainable measures. Due to their broad spectrum of C-H oxyfunctionalization reactions, fungal unspecific peroxygenases (UPOs) are becoming important biotechnological tools in organic synthesis while their potential use in biodegradation processes should not be underestimated. Here, we present a colorimetric screening assay aimed at engineering UPOs for the degradation of epoxy resins. We based our study on Hexflow® RTM-6, a commercial epoxy resin used extensively in the aeronautics sector. UPO mutants from the short and long families were initially benchmarked by GC/MS to determine their potential