AUTHOR=Hilberath Thomas , van Troost Anouska , Alcalde Miguel , Hollmann Frank 

TITLE=Assessing Peroxygenase-Mediated Oxidations in the Presence of High Concentrations of Water-Miscible Co-Solvents

JOURNAL=Frontiers in Catalysis

VOLUME=2

YEAR=2022

URL=https://www.frontiersin.org/journals/catalysis/articles/10.3389/fctls.2022.882992

DOI=10.3389/fctls.2022.882992

ISSN=2673-7841

ABSTRACT=<p>The use of water-miscible organic co-solvents in biocatalysis is a simple procedure for obtaining higher enzymatic activities toward hydrophobic substrates. However, effects on activity and stability have to be carefully evaluated, also with regard to the type and concentration of the respective co-solvent. In this contribution, we investigated and evaluated the effect of some common water-miscible co-solvents on the biocatalytic performance of the recombinant unspecific peroxygenase r<italic>Aae</italic>UPO from <italic>Agrocybe aegerita</italic>. r<italic>Aae</italic>UPO showed promising activities in the presence of high concentrations of the best co-solvent acetonitrile, which enabled to use higher substrate concentrations (≥100 mM). Employing high acetonitrile concentrations for UPO-mediated oxidation of ethylbenzene to (<italic>R</italic>)-1-phenylethanol was demonstrated under preparative scale conditions and led to product accumulation rates of 31 mM h<sup>−1</sup>.</p>